MALDI & ESI & LC ESI QQTOF AND LC ESI LTQ-ORBITRAP MS TRAINING

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的实验室广泛使用Applied Biosystems API QSTAR（tm）Pulsar i QqTOF质谱仪，用于表征从合作研究项目中获得的复杂生物样品。在本报告年度，几名工作人员接受了通过ESI-MS/MS、MALDI-MS/MS和LC-ESI-MS/MS分析样品的QSTAR设计和使用培训。他们接受了使用批参数文件、处理脚本和针对典型蛋白质分析优化的自动IDA MS/MS功能的培训。提供了液相色谱教学，包括柱填充方法学的理论和应用，液相色谱分离参数和ESI接口。前资源工作人员Seward博士准备了一份关于仪器操作和数据处理的优秀PowerPoint指南，当他们开始使用这些系统时，将分发给所有小组成员。在2007年春季安装Thermo-Fisher LTQ-Orbitrap MS系统后，Leymarie博士和Infusini博士开始负责该系统，参加了Thermo培训课程，并开始向其他小组成员介绍其硬件和软件功能。Infusini博士完成了在BUSM的工作，并于2009年秋季移居澳大利亚，他的职位由Voillard博士担任。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our laboratory makes extensive use of the Applied Biosystems API QSTAR(tm) Pulsar i QqTOF mass spectrometers for the characterization of complex biological samples obtained from collaborative research projects. During the current reporting year, several staff members have been provided training on the design and use of the QSTAR for the analysis of samples via ESI-MS/MS, MALDI-MS/MS and LC-ESI-MS/MS. They have been trained in the use of batch parameter files, processing scripts and automated IDA MS/MS functions that have been optimized for typical protein digests. LC instruction has been provided and includes theory and application of column packing methodology, LC separation parameters and ESI interfaces. Former Resource staff member Dr. Seward prepared an excellent powerpoint guide to instrument operation and data processing that is distributed to all group members when they are beginning to use these systems. After the Thermo-Fisher LTQ-Orbitrap MS system was installed in the spring of 2007, Drs. Leymarie and Infusini took on responsibility for the system, attended the Thermo training course and began to introduce other group members to its hardware and software feaures. Dr. Infusini completed his work at BUSM and moved to Australia in fall 2009 and his role has been taken on by Dr. Voillard.