EVOLUTION AND EMERGENCE OF SIMIAN RETROVIRUSES IN BANGLADESH

孟加拉国猴逆转录病毒的进化和出现

• 批准号: 8172777
• 负责人: LISA E JONES-ENGEL
• 金额: $ 15.51万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172777
• 关键词: Area; Bangladesh; Complement; Computer Retrieval of Information on Scientific Projects Database; Country; Evolution; Funding; Genetic Recombination; Geographic Locations; Grant; Human; Individual; Infectious Agent; Institution; Langurs; Macaca; Measures; Phylogenetic Analysis; Population; Prevalence; Recording of previous events; Research; Research Personnel; Resources; Retroviridae; Retroviridae Infections; Sampling; Seroprevalences; Simian Foamy Virus; Simian Retroviruses; Site; Source; Time; Type D Retrovirus; United States National Institutes of Health; Virus; cohort; enzootic; epizootiology; genetic analysis; human population study; nonhuman primate; transmission process

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Multiple NHP (nonhuman primate) species, including 5 species of macaques and 3 species of langurs, coexist with humans in different areas and ecological contexts in Bangladesh, one of the most densely populated countries in the world. Interaction among populations of NHPs as well as between humans and NHPs increases the likelihood of cross-species transmission of infectious agents. Our 5 year R01 project in Bangladesh is characterizing the epizootiology and evolution of two enzootic simian retroviruses, simian foamy virus (SFV) and simian type D retrovirus (SRV) in macaques and langurs where they occur in a variety of geographic areas and contexts throughout Bangladesh. The initial proposal identified 20 sites that have varying degrees of contact between humans and NHPs, diverse ecological conditions and from 1 to 6 species of NHPs present. Phylogenetic and phylodynamic analyses will elucidate how these viruses have changed over time through co-evolution with their hosts, recombination and host switches. These studies will be complemented by studies on the demographic histories of non-human primate populations. We are also measuring the prevalence of SFV and SRV infection in human populations. We are starting the second year of our project. We have started to assemble a cohort of SFV positive humans in Bangladesh. 14/177 humans that we have sampled to date are seropositive for SFV, the highest seroprevalence of this simian-borne retrovirus in any human population studied to date. We have identified individuals co-infected with SFV and SRV. We have also identified individuals infected with more than one strain of SFV. Genetic analysis has revealed that the SRV detected in the free-ranging NHPs in Bangladesh is not any of the known simian type D retroviruses 1-5. Further research will elucidate the diversity of SRV in NHP populations in Bangladesh, and may demonstrate new, heretofore undescribed retroviruses.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在世界上人口最稠密的国家之一孟加拉国，包括5种猕猴和3种叶猴在内的多种NHP（非人类灵长类）物种与人类在不同的地区和生态环境中共存。NHP群体之间以及人类与NHP之间的相互作用增加了传染源跨物种传播的可能性。我们在孟加拉国的5年R 01项目是表征两种地方性猿猴逆转录病毒，猿猴泡沫病毒（SFV）和猿猴D型逆转录病毒（SRV）在猕猴和叶猴中的流行病学和进化，它们发生在孟加拉国的各种地理区域和环境中。最初的提案确定了20个地点，这些地点人类与NHP之间有不同程度的接触，生态条件多样，存在1至6种NHP。系统发育和进化动力学分析将阐明这些病毒如何随着时间的推移通过与宿主的共同进化、重组和宿主转换而发生变化。这些研究将得到非人灵长类种群人口统计学史研究的补充。我们还在测量SFV和SRV在人群中的感染率。我们开始了我们项目的第二年。我们已经开始在孟加拉国聚集一批SFV阳性人群。我们迄今为止采样的177人中有14人对SFV呈血清阳性，这是迄今为止研究的任何人群中这种猿猴传播的逆转录病毒的最高血清阳性率。我们已经确定了同时感染SFV和SRV的个体。我们还发现了感染一种以上SFV毒株的个体。遗传分析表明，在孟加拉国自由放养的NHP中检测到的SRV不是任何已知的猿猴D型逆转录病毒1-5。 进一步的研究将阐明孟加拉国NHP人群中SRV的多样性，并可能证明新的，迄今为止未描述的逆转录病毒。