TAS::75 0872::TAS THE JOHNS HOPKINS PROTEOMIC INNOVATION CENTER IN HEART FAILURE

塔斯马尼亚州::75 0872::塔斯马尼亚州约翰霍普金斯大学心力衰竭蛋白质组学创新中心

• 批准号: 8537322
• 负责人: Jennifer E Van Eyk
• 金额: $ 230万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2015-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8537322
• 关键词: Animal Model; Bioinformatics; Biological; Cardiac; Cardiac Myocytes; Cell model; Cell surface; Cells; Clinical; Communities; Data; Goals; Heart failure; Human; Human Resources; Mass Spectrum Analysis; Methods; Modeling; Pathway interactions; Patients; Peptides; Plasma; Post Translational Modification Analysis; Post-Translational Protein Processing; Production; Protein Isoforms; Proteins; Proteome; Proteomics; Resources; Risk Marker; Sampling; Stratification; Technology; Universities; base; diabetic cardiomyopathy; human disease; innovation; multiple reaction monitoring; novel; tool

The goal of the Johns Hopkins University Proteomic Center is to employ innovative proteomic technologies to pursue compelling biological and clinical questions in heart failure. Data obtained from projects within this center will contribute towards the expansion of an integrated cell based model to facilitate understanding of pathways and subproteome networks. The integrated approach will assist in building testable hypothesis which will be accepted or rejected based on the use of targeted quantitative mass spectrometry based methods in human disease samples to verify/determine the complementarities between animal models and human disease. To accomplish this, absolute quantitative data on key proteins and their specific isoforms from human samples will be obtained through a novel mass spectrometry based method called multiple reaction monitoring.

约翰霍普金斯大学蛋白质组学中心的目标是采用创新的蛋白质组学技术来研究心力衰竭中引人注目的生物学和临床问题。从该中心的项目中获得的数据将有助于扩展基于细胞的综合模型，以促进对途径和亚蛋白质组网络的理解。该综合方法将有助于建立可检验的假设，该假设将基于在人类疾病样本中使用基于靶向定量质谱的方法来接受或拒绝，以验证/确定动物模型和人类疾病之间的互补性。为了实现这一目标，将通过一种称为多反应监测的基于质谱的新方法获得人体样本中关键蛋白质及其特定亚型的绝对定量数据。