TAS::75 0872::TAS THE JOHNS HOPKINS PROTEOMIC INNOVATION CENTER IN HEART FAILURE

约翰霍普金斯大学蛋白质组学中心的目标是采用创新的蛋白质组学技术来研究心力衰竭的生物学和临床问题。从该中心的项目中获得的数据将有助于扩展基于细胞的综合模型，以促进对途径和亚蛋白质组网络的理解。综合方法将有助于建立可检验的假设，根据在人类疾病样本中使用基于靶向定量质谱的方法来验证/确定动物模型与人类疾病之间的互补性，这些假设将被接受或拒绝。为了实现这一目标，将通过一种称为多重反应监测的新型质谱法获得人类样本中关键蛋白质及其特定亚型的绝对定量数据。

The goal of the Johns Hopkins University Proteomic Center is to employ innovative proteomic technologies to pursue compelling biological and clinical questions in heart failure. Data obtained from projects within this center will contribute towards the expansion of an integrated cell based model to facilitate understanding of pathways and subproteome networks. The integrated approach will assist in building testable hypothesis which will be accepted or rejected based on the use of targeted quantitative mass spectrometry based methods in human disease samples to verify/determine the complementarities between animal models and human disease. To accomplish this, absolute quantitative data on key proteins and their specific isoforms from human samples will be obtained through a novel mass spectrometry based method called multiple reaction monitoring.