TAS::75 0872::TAS THE JOHNS HOPKINS PROTEOMIC INNOVATION CENTER IN HEART FAILURE

约翰霍普金斯大学蛋白质组学中心的目标是利用创新的蛋白质组学技术在心力衰竭方面探索引人注目的生物学和临床问题。从该中心内的项目中获得的数据将有助于扩展基于细胞的综合模型，以促进对通路和亚蛋白质组网络的理解。综合方法将有助于建立可检验的假说，该假说将基于在人类疾病样本中使用基于目标定量质谱学的方法来验证/确定动物模型和人类疾病之间的互补性而被接受或拒绝。为了实现这一目标，将通过一种名为多反应监测的新的基于质谱学的方法从人体样本中获得关键蛋白质及其特定亚型的绝对定量数据。

The goal of the Johns Hopkins University Proteomic Center is to employ innovative proteomic technologies to pursue compelling biological and clinical questions in heart failure. Data obtained from projects within this center will contribute towards the expansion of an integrated cell based model to facilitate understanding of pathways and subproteome networks. The integrated approach will assist in building testable hypothesis which will be accepted or rejected based on the use of targeted quantitative mass spectrometry based methods in human disease samples to verify/determine the complementarities between animal models and human disease. To accomplish this, absolute quantitative data on key proteins and their specific isoforms from human samples will be obtained through a novel mass spectrometry based method called multiple reaction monitoring.