CORALE-SeroNet Immune Bioanalytics Core

摘要 免疫生物分析核心(IBC)将作为我们U54 SARS-CoV-2的关键共享资源核心 血清学卓越中心。这种集中式资源将提供最先进的基础设施 以及在高分辨率、定向质谱学方面的专业知识，可用于超过500个的可靠和精确的测量 免疫介体--包括免疫调节蛋白、急性期蛋白、细胞因子和二十烷类化合物-- 通过数以千计的人类生物样本。该IBC将负责自动化样品处理和 管理一系列样品类型、蛋白质和代谢物提取、质谱学和生化 免疫因素分析、复杂的数据集成和数据传输，以支持U54项目。作为以下内容的一部分 更大的SeroNet U54卓越中心，IBC将进一步提供测量能力和专业知识 为所有SeroNet研究人员提供免疫调节肽和二十烷基类因子，以及提供 协助数据标准化和跨中心集成。

ABSTRACT The Immune Bioanalytics Core (IBC) will serve as a critical Shared Resource Core for our U54 SARS-CoV-2 Serological Sciences Center of Excellence. This centralized resource will provide state-of-the-art infrastructure and expertise in high resolution, directed mass spectrometry for the robust and precise measure of over 500 immune mediators - including immune-modulatory proteins, acute phase proteins, cytokines and eicosanoids – across thousands of human biosamples. This IBC will be responsible for automated sample handling and curation for a number of sample types, protein and metabolite extraction, mass spectrometry and biochemical assay of immune factors, complex data integration, and data transfer, in support of the U54 Projects. As part of the larger SeroNet U54 Centers for Excellence, the IBC will further provide capacity and expertise for measure of immune-modulatory peptides and eicosanoids factors for all SeroNet investigators, as well as provide close assistance with data standardization and cross-Center integration.