Genomics and Proteomic Core

A.1.核心的总体目标 核心C，基因组和蛋白质组核心（以前的核心C）的主要目的是继续为主要研究者提供尖端发现基因组学和蛋白质组学工具，以评估和表征许多犬和鼠起搏模型以及人类心脏活检样本。基本上是这样。核心C将提供至关重要的实验服务，这是每个项目成功不可或缺的。它是一个集中的设施，用于分析，编辑和定量水平的mRNA，microRNA和一些关键的亚蛋白质组和蛋白质的相关性。蛋白质组学小组还将提供大量翻译后修饰（PTM）的详细分析，使用多反应监测（MRM）验证基因组数据，新的基于质谱（MS）的定量方法沿着其他MS技术。 此外，核心有一个集成的细胞建模组件，将执行计算建模，以完善和进一步开发DHF和CRT的工作细胞模型。

A.1. General Goals of the Core The primary purpose of Core C, the genomic and proteomic core (previously Core C) will be to continue providing the principle investigators with cutting edge discovery genomics and proteomics tools to assess and characterize a number of canine and murine pacing models as well as human heart biopsy samples. In essence. Core C will provide crucial experimental services that are integral to the success of each project. It is a centralized facility for the analysis, compilation and correlation of quantitative levels of mRNA, microRNA and a number of key subproteomes and proteins. The proteomics group will also provide detailed analysis of a large number of post-translational modifications (PTM), validation of genomic data using multiple reaction monitoring (MRM), new mass spectrometry (MS) -based quantitative methods along with other MS techniques. In addition, the core has an integrated cellular modeling component that will perform computational modeling to refine and further develop a working cellular model of DHF and CRT.