CORE BIOLOGY AND SYNTHESIS FACILITIES

核心生物学和合成设施

• 批准号: 8360594
• 负责人: MUKUND P. SIBI
• 金额: $ 52.94万
• 依托单位: NORTH DAKOTA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-09-23
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360594
• 关键词: Biological Assay; Biology; Centers of Research Excellence; Consultations; Core Facility; Disease; Equipment; Experimental Designs; Faculty; Funding; Goals; Grant; Histone Deacetylase Inhibitor; Human Resources; Human Resources Development; Lead; Matrix Metalloproteinase Inhibitor; Molecular Biology; National Center for Research Resources; North Dakota; Peptide Hydrolases; Play; Principal Investigator; Qualifying; Reagent; Research; Research Infrastructure; Research Personnel; Resources; Source; Structure-Activity Relationship; Training; United States National Institutes of Health; analog; cost; design; drug discovery; efficacy testing; in vitro Bioassay; success; tissue culture; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Center views the activities of the core facilities to be central to the success of its scientific and human resource development goals. The main objectives are to maintain the activity of the Core Biology Facility and establish a state-of-the-art Core Synthetic Facility that will impact research at NDSU and beyond. These facilities will provide training for researchers, consultation in experimental design, and function as a resource for equipment. The qualified personnel in these core facilities will assist junior investigators and will also play a key role in assisting other NDSU researchers and faculty in the state of North Dakota. The Core Biology Facility was established in 2003. It is comprised of three components: Tissue Culture, Molecular Biology, and Bioassays, and offers training and use of equipment and reagents to researchers. Additionally, the lab is staffed with personnel to perform routine bioassays and in vitro biological assays. The facility routinely performs the essential testing for the efficacy of the MMP and HDAC inhibitors designed by the original COBRE investigators. The establishment of a Synthetic core is intended to aid all biomedical researchers by providing small organic molecules for research tools and to increase the throughput of current drug discovery endeavors in the Center. A facility that can provide rapid and parallel synthesis of many analogs of a lead compound is crucial for understanding structure activity relationships and developing new directions.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该中心认为，核心设施的活动是其科学成功的核心。 人力资源开发目标。主要目标是维持 核心生物设施，并建立一个国家的最先进的核心合成设施，将影响 研究在NDSU和超越。这些设施将为研究人员提供培训， 在实验设计中，并作为设备的资源。合格人员在 这些核心设施将协助初级调查人员，并在协助其他 NDSU研究人员和教师在北达科他州。 核心生物学设施成立于2003年。它由三部分组成：组织 培养，分子生物学和生物测定，并提供培训和使用设备， 研究人员的试剂。此外，实验室配备了人员， 生物测定和体外生物测定。该设施定期进行必要的测试， 由最初的COBRE研究者设计的MMP和HDAC抑制剂的疗效。 合成核心的建立旨在通过为研究工具提供小有机分子来帮助所有生物医学研究人员，并增加中心当前药物发现工作的吞吐量。一种能够快速、平行合成多种先导化合物类似物的设备对于理解构效关系和开发新方向至关重要。