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Flow Cytometry

流式细胞仪

基本信息

批准号：
8300287
负责人：
MAX S. WICHA
金额：
$ 13.98万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-06-01 至 2017-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8300287
关键词：
Accounting Antibodies Applications Grants Basic Science Calendar Cancer Biology Cancer Center Support Grant Cell Separation Cells Characteristics Client Clinical Clinical Sciences Comprehensive Cancer Center Consultations Core Facility Data Data Set Detection Development Disease Evaluation Event Experimental Designs Fee-for-Service Plans Flow Cytometry Fluorescent Dyes Grant Hour Human Immune response Immunofluorescence Immunologic Institution Investigation Isotropy Laboratories Lasers Light Malignant - descriptor Measurement Measures Medical Michigan Pathogenesis Phenotype Population Premalignant Process Publications Research Research Personnel Resources Sampling Science Services Speed Stem cells Surface Antigens Training Universities University of Michigan Comprehensive Cancer Center antibody conjugate base cancer stem cell complex biological systems cytokine discount instrument instrumentation member nephelometry operation particle counter response tumor

项目摘要

The Flow Cytometry Core Core is a fee-for-service facility providing flow cytometry instrumentation and expertise to members of the University of Michigan Comprehensive Cancer Center (UM-CCC). The Flow Core has been in continuous operation since 1964 with the main facility residing in dedicated laboratory space provided by the Comprehensive Cancer Center since 1997, For calendar year 2010 there were 95 UM-CCC-affiliated members using the facility, accounting for 51% of the core user base (187 total users) while accounting for 82% of total flow core usage. The Flow Core also provides access to all UM investigators in a broad range of basic and medical sciences. Investigators deliver pre-processed samples to the Core for flow cytometric analysis and/or cell sorting. The core operates on a fee-for-service basis, with a recharge rate of $120/hour. Comprehensive Cancer Center Investigators are able to bill 50% of their usage directly to the CCC core support grant. Recharge rates are further discounted for those investigators willing to provide self-operation on unstaffed Instrumentation. Instruments are operated by trained flow cytometry operators (7.6 FTEs). Access to the facility Is reserved in advance (24-hour minimum) on a first-come basis. Core instrumentation includes one Coulter MoFlo XDP multilaser high-speed ceil sorter, three BD Biosciences FACSAria multilaser high-speed cell sorters, two BD Biosciences FACSDIVa multilaser high-speed cell sorters, a Coulter Cyan multilaser analzer, two BDBiosciences FACSCanto II analyzers, two BD Biosciences FACSCalibur multilaser analyzers, and a Coulter Z2 Particle Counter.

流式细胞仪核心是一个收费的服务设施，为密歇根大学综合癌症中心（UM-CCC）的成员提供流式细胞仪仪器和专业知识。自1964年以来，Flow Core一直在持续运行，自1997年以来，主要设施位于综合癌症中心提供的专用实验室空间。2010年，有95个um - cc附属成员使用该设施，占核心用户群（187名用户）的51%，占总流量核心使用量的82%。流动核心还提供访问所有UM研究人员在广泛的基础和医学科学。