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Flow Cytometry

流式细胞仪

基本信息

批准号：
8300287
负责人：
MAX S. WICHA
金额：
$ 13.98万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-06-01 至 2017-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8300287
关键词：
Accounting Antibodies Applications Grants Basic Science Calendar Cancer Biology Cancer Center Support Grant Cell Separation Cells Characteristics Client Clinical Clinical Sciences Comprehensive Cancer Center Consultations Core Facility Data Data Set Detection Development Disease Evaluation Event Experimental Designs Fee-for-Service Plans Flow Cytometry Fluorescent Dyes Grant Hour Human Immune response Immunofluorescence Immunologic Institution Investigation Isotropy Laboratories Lasers Light Malignant - descriptor Measurement Measures Medical Michigan Pathogenesis Phenotype Population Premalignant Process Publications Research Research Personnel Resources Sampling Science Services Speed Stem cells Surface Antigens Training Universities University of Michigan Comprehensive Cancer Center antibody conjugate base cancer stem cell complex biological systems cytokine discount instrument instrumentation member nephelometry operation particle counter response tumor

项目摘要

The Flow Cytometry Core Core is a fee-for-service facility providing flow cytometry instrumentation and expertise to members of the University of Michigan Comprehensive Cancer Center (UM-CCC). The Flow Core has been in continuous operation since 1964 with the main facility residing in dedicated laboratory space provided by the Comprehensive Cancer Center since 1997, For calendar year 2010 there were 95 UM-CCC-affiliated members using the facility, accounting for 51% of the core user base (187 total users) while accounting for 82% of total flow core usage. The Flow Core also provides access to all UM investigators in a broad range of basic and medical sciences. Investigators deliver pre-processed samples to the Core for flow cytometric analysis and/or cell sorting. The core operates on a fee-for-service basis, with a recharge rate of $120/hour. Comprehensive Cancer Center Investigators are able to bill 50% of their usage directly to the CCC core support grant. Recharge rates are further discounted for those investigators willing to provide self-operation on unstaffed Instrumentation. Instruments are operated by trained flow cytometry operators (7.6 FTEs). Access to the facility Is reserved in advance (24-hour minimum) on a first-come basis. Core instrumentation includes one Coulter MoFlo XDP multilaser high-speed ceil sorter, three BD Biosciences FACSAria multilaser high-speed cell sorters, two BD Biosciences FACSDIVa multilaser high-speed cell sorters, a Coulter Cyan multilaser analzer, two BDBiosciences FACSCanto II analyzers, two BD Biosciences FACSCalibur multilaser analyzers, and a Coulter Z2 Particle Counter.

流式细胞术核心核心是一个按服务收费的设施，为密歇根大学综合癌症中心 (UM-CCC) 的成员提供流式细胞术仪器和专业知识。 Flow Core 自 1964 年以来一直持续运行，主要设施自 1997 年以来一直位于综合癌症中心提供的专用实验室空间中。2010 日历年，有 95 名 UM-CCC 附属成员使用该设施，占核心用户群（187 名用户总数）的 51%，同时占 Flow Core 使用总量的 82%。 Flow Core 还为所有 UM 研究人员提供了广泛的基础科学和医学科学领域的访问权限。研究人员将预处理的样品传送至 Core 进行流式细胞术分析和/或细胞分选。该核心以按服务收费的方式运行，充电费率为 120 美元/小时。综合癌症中心研究人员可以将其使用量的 50% 直接计入 CCC 核心支持补助金。对于那些愿意在无人值守的仪器上进行自助操作的调查员，充值费率会进一步折扣。仪器由经过培训的流式细胞仪操作员 (7.6 FTE) 操作。使用该设施需提前（至少 24 小时）按照先到先得的原则进行预订。核心仪器包括一台 Coulter MoFlo XDP 多激光高速细胞分选仪、三台 BD Biosciences FACSAria 多激光高速细胞分选仪、两台 BD Biosciences FACSDIVa 多激光高速细胞分选仪、一台 Coulter Cyan 多激光分析仪、两台 BDBiosciences FACSCanto II 分析仪、两台 BD Biosciences FACSCalibur 多激光分析仪分析仪和 Coulter Z2 粒子计数器。