Tissue and Molecular Pathology

组织和分子病理学核心(TMPC)由几个不同的、但高度互动的服务和库组成，所有这些都与癌症研究组织的采购、处理和评估有关(见图2)。(见下文)。组织采购处从手术标本中收集和分发多余的人体组织。患者同意使用他们的组织，这些组织可以根据研究人员的特定需求以各种方式收集和处理。组织学和免疫组织化学服务提供专家组织学和免疫组织化学服务。该实验室使用了几种自动化免疫组织化学仪器，并提供具有各种抗原检索方案和滴定的新抗体的完整工作。组织微阵列服务构建常规和定制设计的组织微阵列，用于免疫组织化学、免疫荧光和S/傅杂交分析。匿名石蜡库由2500多块正常组织和所有类型的肿瘤石蜡切片组成，这些石蜡切片来自多余的手术组织。Core和UMCCC的研究人员使用来自该银行的组织进行所有类型的研究活动，主要是组织微阵列构建和抗体的免疫组织化学研究。激光捕获显微切割服务协助调查人员进行激光捕获显微切割的各个方面。冷冻肿瘤库在可用时收集多余的人体组织，目前包含超过7500个冷冻组织样本，分子病理学研究实验室提供全面的分子病理学分析菜单，重点是评估冷冻和固定的肿瘤人体组织。总体而言，组织和分子病理学核心的服务为UMCCC调查人员提供了各种高质量的关键任务组织采购和评估服务

The Tissue and Molecular Pathology Core (TMPC) consists of several distinct, yet highly interactive services and banks, all related to the procurement, processing and evaluation of tissue for cancer research (see Fig. below). The Tissue Procurement Service collects and distributes surplus human tissue from surgical specimens. Patients are consented for use of their tissues, which can be collected and processed in a variety of ways depending on the specific needs of the investigator. The Histology and immunohistochemistry Service provides expert histology and immunohistochemistry services. The lab uses several automated Immunohistochemistry instruments and offers complete workup of novel antibodies with various antigen retrieval protocols and titrations. The Tissue Microarray Service constructs routine and custom designed tissue microarrays to be used in immunohistochemical, immunofluorescent and in s/fu hybridization assays. The Anonymous Paraffin Bank consists of over 2500 paraffin blocks of normal tissues and tumors of all types collected from surplus surgical tissue. Tissues from this bank are used by the Core and UMCCC Investigators for all types of research activities, primarily tissue microarray construction and immunohistochemical workup of antibodies. The Laser Capture Microdissection Service assists investigators with all aspects of laser capture microdissection. The Frozen Tumor Bank collects surplus human tissue when available, and currently contains over 7500 frozen tissue samples, The Molecular Pathology Research Laboratory offers a full menu of molecular pathology assays with a focus on evaluating frozen and fixed neoplastic human tissues. Collectively, the services of the Tissue and Molecular Pathology Core provide UMCCC investigators access to a wide variety of high-quality mission-critical tissue procurement and evaluation services