Protein Engineering and Expression Core

蛋白质工程和表达核心

• 批准号: 8215971
• 负责人: Joseph DiDonato
• 金额: $ 26.18万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2015-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8215971
• 关键词: Alleles; Biological Assay; Cardiovascular Diseases; Enzymes; Generations; Inflammation; Laboratories; Phosphatidylcholine-Sterol O-Acyltransferase; Productivity; Protein Engineering; Proteins; Scientist; Serum Proteins; Structural Protein; Training; Work; biophysical chemistry; computational chemistry; human NOS3 protein; instrumentation; interest; member; mutant; oxidation; programs; protein expression; protein purification

C. Description of Core Unit: C.1. Rationale and Objectives Each of the three laboratories involved in the Program Project will express distinct classes of proteins from serum proteins (ApoA1) and enzymes (PON1, LCAT, and MPO) to cytoplasmic ribosomal structural proteins (like L13a), enzymes (eNOS, nNOS, and Akt) and mutant forms of a subset of these in order to perform subsequent enzymatic assays as described in the Projects and analysis utilizing diverse and sophisticated instrumentation detailed in the Biophysical and Computational Chemistry Core (Core C). The principal objective of the Protein Engineering and Expression Core is to provide a centralized means for high volume protein expression thereby facilitating the relatively sophisticated and diverse biophysical studies on the proteins of interest for each Project The Core will also function as an educational facility for training members of the Program Project laboratories on ways to optimize protein expression using various strategies and also in the generation of mutants of specific alleles desired for protein expression. Training in the purification of proteins will also be provided by the Core. Centralizing these aspects of the work and training is expected to greatly enhance efficiency and productivity of the scientists participating in each project.

C.核心单元描述：