AB/SCIEX QTrap 6500 Mass Spectrometer Metabolic profiling system

AB/SCIEX QTrap 6500 质谱仪代谢分析系统

• 批准号: 8640472
• 负责人: Joseph DiDonato
• 金额: $ 60万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640472
• 关键词: Affect; Amino Acid Sequence; Biological Markers; Clinic; Clinical; Commit; Custom; Development; Disease; Distant; Foundations; Funding; Health; High Pressure Liquid Chromatography; Human; Human Resources; Hybrids; Institution; Ions; Laboratories; Maintenance; Mass Spectrum Analysis; Metabolic; National Center for Research Resources; Ohio; Peptide Sequence Determination; Productivity; Program Research Project Grants; Research; Research Personnel; Research Project Grants; Resources; Sampling; Schedule; Sensitivity and Specificity; Services; System; Technology; Time; Travel; United States National Institutes of Health; Universities; analytical tool; instrument; instrumentation; mass spectrometer; member; metabolomics; operation; programs; small molecule; translational study

DESCRIPTION (provided by applicant): The purpose of this proposal is to seek funds for the purchase of a hybrid triple quadrupole/linear ion trap (AB Sciex QTRAP 6500) mass spectrometry metabolomics profiling system with a multiplexed MPX2 HPLC interface. This instrument will expand and upgrade mass spectrometry capabilities within the Northeastern Ohio region, advancing research programs within the Cleveland Clinic Foundation (CCF) and its partner institution, Case Western Reserve University (CWRU) other Cleveland CTSA member institutions and Cleveland State University (CSU). Major Users all have NIH-funded research grants, including recent multiple Program Project Grants and R01's, all which have Projects and studies that are currently conducted using mass spectrometry to ask, investigate and answer key questions concerning major diseases that affect human health. Due to the full utilization of available instrumentation in the region, many Investigators now have to schedule time many months in advance to obtain access to this technology and/or travel to distant institutions. As detailed in the application, supported projects primarily include biomarker discovery and clinical/translational studies nearly all of which also utilize the NCRR-funded Cleveland CTSA. The requested mass spectrometer provides the high through-put capabilities that will assure timely access to this type of instrumentation, while not sacrificing versatility, sensitivity and specificity required for these projects. This analytical activity will be conducted within our existing Small Molecule Mass Spectrometry Core, which provides support to investigators at CCF, CWRU, CSU and neighboring institutions by performing structural elucidation studies, biomarker quantification, protein sequencing/identification, and related services. Mass spectrometry is increasingly being used as an analytical tool by CCF and partner institution investigators, with demand greatly outpacing availability, resulting in significant delays in access, and hampered productivity. The acquisition of the requested mass spectrometer is essential to alleviate the backlog of samples waiting to be analyzed. CCF has committed substantial resources to the development of mass spectrometry in the region, including custom renovation of laboratory space for Small Molecule Mass Spectrometry Core, personnel to oversee its operation, and a fiscal plan to insure its operation and maintenance for the long-term.

描述（由申请人提供）：该提案的目的是寻求资金购买混合三倍四极杆/线性离子陷阱（AB SCIEX QTRAP 6500）质谱代谢组学分析系统，并具有多重MPX2 HPLC界面。该工具将扩大和升级俄亥俄州东北部地区的质谱能力，推进克利夫兰诊所基金会（CCF）内的研究计划及其合作伙伴机构Case Western Reserve University（CWRU）其他克利夫兰CTSA成员机构和克利夫兰州立大学（CSU）。主要用户都拥有NIH资助的研究补助金，其中包括最近的多个计划项目赠款和R01，所有这些都有目前使用质谱法进行的项目和研究，以询问，调查和回答有关影响人类健康的主要疾病的关键问题。由于该地区可用的仪器充分利用，现在许多调查人员必须提前安排时间数月以获取该技术和/或前往遥远机构的访问。如应用程序中所述，受支持的项目主要包括生物标志物发现和临床/翻译研究，几乎所有这些研究都使用了NCRR资助的Cleveland CTSA。请求的质谱仪提供了很高的贯穿能力，可以确保及时访问此类仪器，同时不牺牲这些项目所需的多功能性，敏感性和特异性。这种分析活性将在我们现有的小分子质谱核心内进行，该核心通过进行结构阐明研究，生物标志物量化，蛋白质测序/鉴定和相关服务，为CCF，CWRU，CSU和邻近机构的研究人员提供支持。 CCF和合作伙伴机构研究人员越来越多地将质谱法用作分析工具，需求大大超过了可用性，从而导致访问的延迟和妨碍生产率。获得所需的质谱仪的获取对于减轻等待分析的样品积压至关重要。 CCF已为该地区的质谱法发展提供了大量资源，包括针对小分子质谱核心的实验室空间进行定制翻新，监督其运营的人员以及一项财政计划，以确保其长期运作和维护。

项目成果

Marked elevation in plasma trimethylamine-N-oxide (TMAO) in patients with mitochondrial disorders treated with oral l-carnitine.

• DOI: 10.1016/j.ymgmr.2018.04.005
• 发表时间: 2018-06
• 期刊: Molecular genetics and metabolism reports
• 影响因子: 1.9
• 作者: Vallance HD;Koochin A;Branov J;Rosen-Heath A;Bosdet T;Wang Z;Hazen SL;Horvath G
• 通讯作者: Horvath G