AB/SCIEX QTrap 6500 Mass Spectrometer Metabolic profiling system

AB/SCIEX QTrap 6500 质谱仪代谢分析系统

• 批准号: 8640472
• 负责人: Joseph DiDonato
• 金额: $ 60万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640472
• 关键词: Affect; Amino Acid Sequence; Biological Markers; Clinic; Clinical; Commit; Custom; Development; Disease; Distant; Foundations; Funding; Health; High Pressure Liquid Chromatography; Human; Human Resources; Hybrids; Institution; Ions; Laboratories; Maintenance; Mass Spectrum Analysis; Metabolic; National Center for Research Resources; Ohio; Peptide Sequence Determination; Productivity; Program Research Project Grants; Research; Research Personnel; Research Project Grants; Resources; Sampling; Schedule; Sensitivity and Specificity; Services; System; Technology; Time; Travel; United States National Institutes of Health; Universities; analytical tool; instrument; instrumentation; mass spectrometer; member; metabolomics; operation; programs; small molecule; translational study

DESCRIPTION (provided by applicant): The purpose of this proposal is to seek funds for the purchase of a hybrid triple quadrupole/linear ion trap (AB Sciex QTRAP 6500) mass spectrometry metabolomics profiling system with a multiplexed MPX2 HPLC interface. This instrument will expand and upgrade mass spectrometry capabilities within the Northeastern Ohio region, advancing research programs within the Cleveland Clinic Foundation (CCF) and its partner institution, Case Western Reserve University (CWRU) other Cleveland CTSA member institutions and Cleveland State University (CSU). Major Users all have NIH-funded research grants, including recent multiple Program Project Grants and R01's, all which have Projects and studies that are currently conducted using mass spectrometry to ask, investigate and answer key questions concerning major diseases that affect human health. Due to the full utilization of available instrumentation in the region, many Investigators now have to schedule time many months in advance to obtain access to this technology and/or travel to distant institutions. As detailed in the application, supported projects primarily include biomarker discovery and clinical/translational studies nearly all of which also utilize the NCRR-funded Cleveland CTSA. The requested mass spectrometer provides the high through-put capabilities that will assure timely access to this type of instrumentation, while not sacrificing versatility, sensitivity and specificity required for these projects. This analytical activity will be conducted within our existing Small Molecule Mass Spectrometry Core, which provides support to investigators at CCF, CWRU, CSU and neighboring institutions by performing structural elucidation studies, biomarker quantification, protein sequencing/identification, and related services. Mass spectrometry is increasingly being used as an analytical tool by CCF and partner institution investigators, with demand greatly outpacing availability, resulting in significant delays in access, and hampered productivity. The acquisition of the requested mass spectrometer is essential to alleviate the backlog of samples waiting to be analyzed. CCF has committed substantial resources to the development of mass spectrometry in the region, including custom renovation of laboratory space for Small Molecule Mass Spectrometry Core, personnel to oversee its operation, and a fiscal plan to insure its operation and maintenance for the long-term.

描述（由申请人提供）：本提案的目的是寻求资金购买具有多重 MPX2 HPLC 接口的混合三重四极杆/线性离子阱 (AB Sciex QTRAP 6500) 质谱代谢组学分析系统。该仪器将扩展和升级俄亥俄州东北部地区的质谱能力，推进克利夫兰诊所基金会 (CCF) 及其合作机构、凯斯西储大学 (CWRU)、其他克利夫兰 CTSA 成员机构和克利夫兰州立大学 (CSU) 的研究项目。主要用户均拥有 NIH 资助的研究资助，包括最近的多项计划项目资助和 R01 资助，所有这些资助都有目前使用质谱法进行的项目和研究，以提出、调查和回答有关影响人类健康的重大疾病的关键问题。由于充分利用了该地区现有的仪器，许多研究人员现在必须提前几个月安排时间，以获得这项技术和/或前往遥远的机构。正如申请中详细说明的，支持的项目主要包括生物标志物发现和临床/转化研究，几乎所有这些项目也利用 NCRR 资助的克利夫兰 CTSA。所需的质谱仪提供高通量能力，确保及时访问此类仪器，同时不牺牲这些项目所需的多功能性、灵敏度和特异性。这项分析活动将在我们现有的小分子质谱核心内进行，该核心通过进行结构阐明研究、生物标志物定量、蛋白质测序/鉴定和相关服务，为 CCF、CWRU、CSU 和邻近机构的研究人员提供支持。质谱法越来越多地被 CCF 和合作机构研究人员用作分析工具，但需求远远超过了可用性，导致获取严重延迟，并影响了生产力。获取所需的质谱仪对于缓解等待分析的样品积压至关重要。 CCF 已投入大量资源用于该地区质谱技术的发展，包括小分子质谱核心实验室空间的定制改造、监督其运营的人员以及确保其长期运营和维护的财政计划。

项目成果

Marked elevation in plasma trimethylamine-N-oxide (TMAO) in patients with mitochondrial disorders treated with oral l-carnitine.

• DOI: 10.1016/j.ymgmr.2018.04.005
• 发表时间: 2018-06
• 期刊: Molecular genetics and metabolism reports
• 影响因子: 1.9
• 作者: Vallance HD;Koochin A;Branov J;Rosen-Heath A;Bosdet T;Wang Z;Hazen SL;Horvath G
• 通讯作者: Horvath G