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Scientific Core 1:Human Clinical and Protein Engineering Core

科学核心1：人类临床和蛋白质工程核心

基本信息

批准号：
9049529
负责人：
Joseph DiDonato
金额：
$ 14.96万
依托单位：
CLEVELAND CLINIC LERNER COM-CWRU
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-08-01 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9049529
关键词：
3-nitrotyrosine Advertising Amino Acids Animal Disease Models Antibodies Antigens Aorta Biochemical Biocompatible Materials Biological Assay Blood Vessels Cardiovascular Diseases Cardiovascular system Caring Carotid Arteries Clinical Clinical Engineering Clinical Research Consent DNA Diagnostic Education Endotoxins Funding Generations Goals High Density Lipoproteins Human In Vitro Individual Inflammation Institutional Review Boards Lipoprotein (a)Lipoproteins Low-Density Lipoproteins Lysine Molecular Molecular Genetics Monoclonal Antibodies Myocardial Infarction Names Patients Peripheral Phenotype Plasma Prevention Protein Engineering Proteins Recombinant Proteins Registries Research Research Personnel Resources Sampling Serum Services Site Source Specimen Structure Students Techniques Tissues Training Training Programs Training and Education Transfer RNA Venous blood sampling Very low density lipoprotein Whole Blood Work abstracting clinical material clinically relevant clinically significant cohort design follow-up human biological material in vivo innovation interest liver biopsy lung injury monocyte mutant myocardial biopsy oxidation polyclonal antibody programs protein expression repository translational study unnatural amino acids volunteer

项目摘要

Scientific Core 1(SC1). Project Summary/Abstract Each of the three Projects within the Program Project will require purified native and oxidatively modified lipoproteins, access to human clinical material with known clinical follow-up and a source of native, mutant or site-specific post-translationally modified proteins of interest to use in their studies. The Human Clinical Material & Protein Engineering Core (Scientific Core 1; SC1) will provide all of these services, allowing for expansion of the scope, scale and clinical significance of proposed studies in each Project of this Program. The Human Clinical Material & Protein Engineering Core has 3 primary objectives: First, the core will facilitate human clinical and translational studies of Program investigators by providing the following services: (i) Coordination of all IRB paperwork, advertising / consenting of volunteers, contact of patients with specific phenotypes (e.g. high LP(a) levels), and phlebotomy services. (ii) Using standardized SOP for isolation and characterization of the following human biological materials: whole blood (e.g. for PMN and MPO isolation); plasma; serum; isolated lipoproteins (e.g. VLDL, LDL, HDL and LP(a)). (iii) Coordination of monocyte isolation via elutriation. (iv) Coordination of access to archival human clinical specimens (plasma, serum, DNA) from the GeneBank cohort for proposed clinical studies. (v) Access to human clinical specimens available within the Center for Cardiovascular Diagnostics & Prevention. Second, the core will assist Program investigators with protein engineering and antibody generation by providing the following services: (i) assist investigators with design, expression, and purification of distinct classes of wild type and mutant forms of proteins. (ii) Generation of low endotoxin recombinant proteins for in vivo studies. (iii) Orthogonal amino acid incorporation using tRNA mutants to specifically form individual recombinant proteins harboring unnatural amino acids (e.g. nitrotyrosine, chlorotyrosine, Nε-acetyl-lysine). And (iv) assistance in generation of both polyclonal and monoclonal antibodies. Finally, the core will serve as an educational and training hub for Program investigators, especially students and fellows. Centralizing these aspects of the work and training will greatly enhance the significance, innovation, and clinical relevance of studies performed in each Project of this Program.

科学核心1（SC1）。项目总结/文摘