Recombinant Protein Expression and Molecular Cloning

重组蛋白表达和分子克隆

• 批准号: 8015699
• 负责人: Joseph DiDonato
• 金额: $ 15.8万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-08 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8015699
• 关键词: Alleles; Biological; Biological Assay; Biophysics; Cloning; Educational process of instructing; Enzymes; Generations; Human Resources; Knock-in Mouse; Laboratories; Mass Spectrum Analysis; Molecular Cloning; Mutagenesis; Phosphatidylcholine-Sterol O-Acyltransferase; Production; Productivity; Proteins; Recombinant Proteins; Research Personnel; Scientist; Serum Proteins; Techniques; Training; Transgenic Mice; Transgenic Organisms; Universities; Work; experience; expression cloning; instrumentation; interest; meetings; member; mutant; programs; protein expression; protein purification

Each of the three laboratories involved in the Program Project will express distinct classes of proteins from serum proteins (ApoAI) to enzymes (LCAT, and MPO) and mutant forms of a subset of these in order to perform subsequent enzymatic assays as described in the Projects and analysis utilizing diverse and sophisticated instrumentation detailed in the Mass Spectrometry and Biophysics Core (Core B). The principal objective ofthe Recombinant Protein Expression and Molecular Cloning Core is to provide a centralized means for high volume protein expression thereby facilitating the relatively sophisticated and diverse biophysical and biological studies on the proteins of interest for each Project, in addition, the Core will also provide molecular cloning/ mutagenesis capabilities to all three Projects. The Core will also function as an educational facility for training members of the Program Project laboratories on ways to optimize protein expression using various strategies and also in the generation of mutants of specific alleles desired for protein expression or for targeting alleles for transgenic mouse production ( CC/CWRU Transgenic mouse Core is located at Case Western Reserve University). Training in the purification of proteins will also be provided by the Core. Centralizing these aspects of the work and training is expected to greatly enhance efficiency and productivity of the scientists participating in each project.

参与该项目的三个实验室将分别表达不同种类的蛋白质