Protein Engineering and Expression Core

蛋白质工程和表达核心

• 批准号: 7793873
• 负责人: Joseph DiDonato
• 金额: $ 15.07万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2014-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793873
• 关键词: Alleles; Biological Assay; Enzymes; Generations; Laboratories; Phosphatidylcholine-Sterol O-Acyltransferase; Productivity; Protein Engineering; Proteins; Scientist; Serum Proteins; Structural Protein; Training; Work; computational chemistry; human NOS3 protein; instrumentation; interest; member; mutant; programs; protein expression; protein purification

C. Description of Core Unit: C.1. Rationale and Objectives Each of the three laboratories involved in the Program Project will express distinct classes of proteins from serum proteins (ApoA1) and enzymes (PON1, LCAT, and MPO) to cytoplasmic ribosomal structural proteins (like L13a), enzymes (eNOS, nNOS, and Akt) and mutant forms of a subset of these in order to perform subsequent enzymatic assays as described in the Projects and analysis utilizing diverse and sophisticated instrumentation detailed in the Biophysical and Computational Chemistry Core (Core C). The principal objective of the Protein Engineering and Expression Core is to provide a centralized means for high volume protein expression thereby facilitating the relatively sophisticated and diverse biophysical studies on the proteins of interest for each Project The Core will also function as an educational facility for training members of the Program Project laboratories on ways to optimize protein expression using various strategies and also in the generation of mutants of specific alleles desired for protein expression. Training in the purification of proteins will also be provided by the Core. Centralizing these aspects of the work and training is expected to greatly enhance efficiency and productivity of the scientists participating in each project.

C.核心单元的描述： C.1。理由和目标 Each of the three laboratories involved in the Program Project will express distinct classes of proteins from serum proteins (ApoA1) and enzymes (PON1, LCAT, and MPO) to cytoplasmic ribosomal structural proteins (like L13a), enzymes (eNOS, nNOS, and Akt) and mutant forms of a subset of these in order to perform subsequent enzymatic assays as described in利用生物物理和计算化学核心（Core C）中详细介绍的多样化和复杂仪器的项目和分析。这 蛋白质工程和表达核心的主要目标是为高量蛋白表达提供一种集中式方法，从而促进了对每个项目感兴趣的蛋白质的相对复杂和多样化的生物物理研究，该核心还将作为培训计划实验室成员的教育设施，以在蛋白质表达方面培训各种蛋白质表达的方式，以使用各种蛋白质表达蛋白质表达，以培养各种蛋白质，以培养各种蛋白质的蛋白质，并在特定的代表中脱颖而出。蛋白质纯化的培训也将由核心提供。预计工作的这些方面和培训将大大提高参与每个项目的科学家的效率和生产力。