Microscopy and Imaging Shared Resource

显微镜和成像共享资源

• 批准号: 8369396
• 负责人: MICHELE CARBONE
• 金额: $ 2.17万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8369396
• 关键词: Animals; Buffers; Calibration; Cancer Center; Cancer Center Support Grant; Caring; Categories; Cells; Complex; Consultations; Equipment; Facility Accesses; Funding; Goals; Growth; Hawaii; Histology; Housing; Image; Imaging technology; Immersion Investigative Technique; Individual; Laboratories; Lasers; Maintenance; Measures; Microdissection; Microscope; Microscopy; Monitor; Oils; Organism; Paper; Pathologist; Research; Research Personnel; Resource Sharing; Scanning; Services; Solutions; Techniques; Time; Tissues; Training; Universities; base; cost; instrument; instrumentation; laser capture microdissection; lens; member; mouse model; operation; repaired; tumorigenesis

Critical to many labs within Cancer Center is the ability to examine tissues and cells at the subcellular level, to count, isolate and measure cells, and to utilize non-invasive techniques to monitor tumorigenesis in mouse models. However, individual labs can rarely afford the state-of-the-art instrumentation required for many of these studies. Based on this need, a microscope core was created in 2002 and administered through the former Laboratory Instrumentation Shared Resource (LISR). The LISR's imaging capabilities have considerably expanded over the years with the acquisition of several important pieces of equipment, including a Leica TCS SP5 laser scanning confocal microscope, a laser capture tissue microdissection instrument, a time-lapse microscope with WeatherStation, and a IVIS Lumina imaging system for live animal studies. As a result of this growth, the former LISR is now the Microscopy and Imaging Share Resource (MISR), which serves as a centralized facility for access to imaging instruments within the Cancer Center. One important function of the MISR is to offer technical support to users and to properly maintain the complex instrumentation housed in the facility through annual maintenance services as well as routine calibrations. Moreover, the facility is responsible for upgrades, repairs, and acquisition of new equipment according to the needs of the cancer center researchers. Non-imaging services previously provided by the LISR, such as freezer monitoring, darkroom maintenance, and repairs of shared centrifuges, are now administered and supported entirely through institutional funds.

对癌症中心内的许多实验室来说，关键是能够在亚细胞水平上检查组织和细胞，计数，分离和测量细胞，并利用非侵入性技术来监测小鼠模型中的肿瘤发生。然而，个别实验室很少能负担得起许多这些研究所需的最先进的仪器。基于这一需求，2002年创建了显微镜核心，并通过前实验室仪器共享资源（LISR）进行管理。LISR的成像能力随着几个重要设备的收购而大大扩展，包括Leica TCS SP5激光扫描共聚焦显微镜，激光捕获组织显微切割仪器，带WeatherStation的延时显微镜和用于活体动物研究的IVIS Lumina成像系统。由于这种增长，以前的LISR现在是显微镜和成像共享资源（MISR），它作为一个集中的设施，用于访问癌症中心内的成像仪器。MISR的一个重要功能是向用户提供技术支持，并通过年度维护服务和例行校准适当维护设施内的复杂仪器。此外，该设施还负责根据癌症中心研究人员的需求进行升级，维修和购买新设备。以前由利比里亚空间研究所提供的非成像服务，如冷冻机监测、暗室维护和共用离心机维修，现在完全由机构资金管理和支助。