Microscopy and Imaging Shared Resource

对于癌症中心内的许多实验室来说，关键是能够在亚细胞水平上检查组织和细胞，计数、分离和测量细胞，并利用非侵入性技术监测小鼠模型中的肿瘤发生。然而，个别实验室很少负担得起许多此类研究所需的最先进的仪器。基于这一需要，2002年创建了一个显微镜核心，并通过前实验室仪器共享资源(LISR)进行管理。多年来，LISR的成像能力得到了极大的扩展，购买了几台重要的设备，包括徕卡TCS SP5激光扫描共聚焦显微镜、激光捕获组织显微解剖仪器、带有WeatherStation的延时显微镜，以及用于活体动物研究的IVIS Lumina成像系统。由于这一增长，以前的LISR现在是显微镜和成像共享资源(MISR)，它是癌症中心内访问成像仪器的中央设施。MISR的一个重要功能是向用户提供技术支持，并通过年度维护服务和例行校准妥善维护设施中的复杂仪器。此外，该设施还负责根据癌症中心研究人员的需求进行升级、维修和购买新设备。以前由LISR提供的非成像服务，如冰柜监测、暗室维护和共用离心机的维修，现在完全由机构资金管理和支持。

Critical to many labs within Cancer Center is the ability to examine tissues and cells at the subcellular level, to count, isolate and measure cells, and to utilize non-invasive techniques to monitor tumorigenesis in mouse models. However, individual labs can rarely afford the state-of-the-art instrumentation required for many of these studies. Based on this need, a microscope core was created in 2002 and administered through the former Laboratory Instrumentation Shared Resource (LISR). The LISR's imaging capabilities have considerably expanded over the years with the acquisition of several important pieces of equipment, including a Leica TCS SP5 laser scanning confocal microscope, a laser capture tissue microdissection instrument, a time-lapse microscope with WeatherStation, and a IVIS Lumina imaging system for live animal studies. As a result of this growth, the former LISR is now the Microscopy and Imaging Share Resource (MISR), which serves as a centralized facility for access to imaging instruments within the Cancer Center. One important function of the MISR is to offer technical support to users and to properly maintain the complex instrumentation housed in the facility through annual maintenance services as well as routine calibrations. Moreover, the facility is responsible for upgrades, repairs, and acquisition of new equipment according to the needs of the cancer center researchers. Non-imaging services previously provided by the LISR, such as freezer monitoring, darkroom maintenance, and repairs of shared centrifuges, are now administered and supported entirely through institutional funds.