LABORATORY OF DEVELOPMENTAL BIOLOGY

发育生物学实验室

• 批准号: 8336885
• 负责人: Ian Amos Glass
• 金额: $ 62.26万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-05-01 至 2016-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8336885
• 关键词: Address; Autopsy; Barker Hypothesis; Biological Assay; Biology; Biomedical Research; Cardiovascular system; Clinical Data; Collection; Congenital Abnormality; Congenital Heart Defects; DNA; Data; Development; Developmental Biology; Dissection; Embryology; Epigenetic Process; Fetal Development; Fetal Tissues; Fetus; Funding; Genetic; Genitourinary system; Genomics; Goals; Grant; Human; Human Development; Image; Imaging technology; Institution; Laboratories; Laboratory Research; Leadership; Molecular; Mutation; Optics; Organ; Patients; Phenotype; Positioning Attribute; Prenatal Diagnosis; Process; RNA; Research; Research Personnel; Research Support; Resources; Sampling; Scanning; Services; Site; Specimen; Staging; System; Techniques; Time; Tissues; United States National Institutes of Health; Universities; Variant; Work; X-Ray Computed Tomography; base; comparative genomic hybridization; cost; fetal; human disease; improved; interest; novel; postnatal; prenatal; tomography; virtual

DESCRIPTION (Provided by Applicant): For 46 years, the Birth Defects Research Laboratory (BDRL) has been the major NIH-funded site for collection and distribution of conceptal tissues. The availability of viable conceptal organs and tissues has made the Laboratory a unique and critical non-profit resource for biomedical research. This application seeks to continue and further develop the core fundamental goal of the laboratory: the systematic collection, staging, identification, and processing of normal specimens and distribution of their tissues to recipients. In this renewal application, we will build upon this central aim to extend the biomedical research resource further by making available samples for DNA/RNA extraction and epigenetic assays. In addition, the investigator will develop the resource by 1) extending the systematic collection, identification, and distribution to abnormal fetuses; 2) correlating prenatal data with the post-termination findings from examination/postmortem; 3) exploiting the virtual histological and phenotyping capabilities of tissue imaging platforms after performing proof-of-principle studies in genitourinary tract and cardiovascular tissues; 4) capitalizing on the expected enrichment of genetic defects underlying fetal congenital anomalies by generating copy number variant data through array-based comparative genomic hybridization studies; 5) systematically making accessible tissues and their data for investigators; and 6) engaging and working with key collaborators to improve services and increase recipient numbers in their respective fields. This application builds upon ARRA support, evaluating the utility of novel tissue imaging systems to enhance BDRL services, and stimulating and supporting research based in part on this resource into the bases of birth defects and normal development. Systematically characterizing abnormal fetuses and distributing tissues from these fetuses will exploit the unique positioning of the BDRL to develop this as a significant research resource and service to researchers who seek to understand the underlying developmental biology of normal and abnormal human development.

描述（由申请人提供）：46年来，出生缺陷研究实验室（BDRL）一直是美国国立卫生研究院资助的收集和分发胎儿组织的主要场所。可行的概念器官和组织的可用性使该实验室成为生物医学研究的独特和关键的非营利性资源。本申请旨在继续并进一步发展实验室的核心基本目标：系统地收集、分期、鉴定和处理正常标本，并将其组织分配给受体。在这个更新申请中，我们将建立在这个中心目标上，通过提供DNA/RNA提取和表观遗传分析的样本，进一步扩展生物医学研究资源。此外，研究者将通过1)将系统的收集、鉴定和分发扩展到异常胎儿来开发资源；2)将产前数据与终止妊娠后的检查/尸检结果相关联；3)在对泌尿生殖系统和心血管组织进行原理验证研究后，利用组织成像平台的虚拟组织学和表型能力；4)通过基于阵列的比较基因组杂交研究生成拷贝数变异数据，利用预期的胎儿先天性异常遗传缺陷的富集；5)系统地为调查人员提供可访问的组织及其数据；6)与主要合作伙伴进行接触和合作，以改善各自领域的服务并增加受助人数。该应用程序建立在ARRA支持的基础上，评估新型组织成像系统的效用，以增强BDRL服务，并刺激和支持部分基于该资源的研究，以了解出生缺陷和正常发育的基础。系统地描述异常胎儿和分布这些胎儿的组织，将利用BDRL的独特定位，将其发展为一个重要的研究资源和服务，为那些寻求了解正常和异常人类发育的潜在发育生物学的研究人员提供帮助。