Bioanalytical/Mass Spectrometry/Proteomics Core E

生物分析/质谱/蛋白质组学 Core E

• 批准号: 8279465
• 负责人: RICHARD M CAPRIOLI
• 金额: $ 11.88万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8279465
• 关键词: Biological Assay; Complex; Consultations; Data Analyses; Data Reporting; Diagnosis; Digestive System Disorders; Disease; Experimental Designs; Facility Accesses; Gel; Goals; Human; Human Resources; Image; Individual; MALDI-TOF Mass Spectrometry; Maintenance; Mass Spectrum Analysis; Molecular; Monitor; Performance; Procedures; Process; Proteins; Proteome; Proteomics; Quality Control; Recording of previous events; Records; Reporting; Research; Research Personnel; Running; Sampling; Services; Students; Tissues; Training; Validation; analytical method; assay development; base; cost effective; gel electrophoresis; instrument; instrumentation; investigator training; liquid chromatography mass spectrometry; mass spectrometer; prevent; small molecule

The Bioanalytical/Mass Spectrometry and Proteomic Core (BMSC) provides cost-effective, state-of-the-art instrumentation and expertise to investigators in the Vanderbilt Digestive Disease Research Center (VDDRC). This core is among the specialized service cores utilizing the technical personnel and instrument facilities located in the Vanderbilt Mass Spectrometry Research Center (MSRC). This core will be used for identification and quantitation of small molecule metabolites and identification and characterization of proteins. The core has been used extensively by 22 VDDRC investigators during the previous five years to assist users in developing analytical methods in experimental design, standard operating procedures, and maintain quality control (QC) records on particular assays, instrument performance, and maintenance history. Core personnel perform assays for investigators and train students and fellows in the theoretical and practical aspects of MS. The MS Core is run as an open-access facility in which users generally prepare their samples and operate the instruments if they so desire. Proteomics samples are submitted to the core for analysis by proteomics staff after consultations between the investigator and core staff have determined the most appropriate class of analytical service. Personnel handle all aspects of sample processing, analysis, and data reporting of samples submitted for proteomics analysis. Administrative staff monitor the use of the instrument facilities by investigators and prepare reports on utilization for use by the Administrative Core. The MSRC has 14 mass spectrometers available to users, in addition to specialized facilities for 2D-differential gel electrophoresis and gel imaging. The Aims of the BMSC are to: 1) provide high-quality GC/MS, tandem LC/MS, and MALDI/TOF mass Spectrometry services for analysis of small molecule metabolites; 2) provide proteomics services for identification and characterization of individual proteins and more complex tissue-specific proteomes; (3) provide analytical expertise in mass Spectrometry for assay development and validation; 4) assist users with data analysis; and 5) provide advanced training in biomedical mass Spectrometry and proteomics to students and fellows. The goal of the Core is to enhance investigator abilities to prevent, diagnose or treat human digestive disease-related disorders.

生物分析/质谱和蛋白质组学核心（BMSC）提供具有成本效益的，最先进的 为范德比尔特消化疾病研究中心的研究人员提供仪器和专业知识 （VDDRC）。该核心是利用技术人员和仪器的专业服务核心之一 位于范德比尔特质谱研究中心（MSRC）的设施。该核心将用于 小分子代谢物的鉴定和定量以及 proteins.在过去的五年里，22名VDDRC调查人员广泛使用该核心， 协助用户开发实验设计中的分析方法，标准操作程序， 维护特定测定、仪器性能和维护的质量控制（QC）记录 历史核心人员为研究人员进行分析，并对学生和研究员进行理论和 MS核心是作为一个开放访问的设施，用户通常准备 他们的样品和操作仪器，如果他们这样做的愿望。蛋白质组学样本提交给核心 在研究者和核心工作人员协商确定后，由蛋白质组学工作人员进行分析 最合适的分析服务。工作人员负责样品处理的各个方面， 分析和提交用于蛋白质组学分析的样品的数据报告。行政人员监测 调查员使用仪器设施的情况，并编写供调查员使用的使用情况报告。 行政核心。MSRC有14台质谱仪可供用户使用，此外还有专门的质谱仪。 用于2D差异凝胶电泳和凝胶成像的设备。BMSC的目标是：1）提供 高质量的GC/MS、串联LC/MS和MALDI/TOF质谱服务，用于分析小型 分子代谢物; 2）为个体的鉴定和表征提供蛋白质组学服务 蛋白质和更复杂的组织特异性蛋白质组;（3）提供质谱分析专业知识 用于分析开发和验证; 4）协助用户进行数据分析;以及5）提供以下方面的高级培训 生物医学质谱和蛋白质组学。核心的目标是增强 研究人员预防、诊断或治疗人类消化系统疾病相关疾病的能力。