Bioanalytical/Mass Spectrometry/Proteomics Core E

生物分析/质谱学和蛋白质组核心(BMSC)提供经济高效、最先进的 向范德比尔特消化疾病研究中心的研究人员提供仪器和专业知识 (VDDRC)。这个核心是利用技术人员和仪器的专业服务核心之一 位于范德比尔特质谱学研究中心(MSRC)的设施。这个核心将用于 小分子代谢物的鉴定和定量及其鉴定和表征 蛋白质。在过去五年中，22名VDDRC调查人员广泛使用了该核心 协助用户开发实验设计的分析方法、标准操作程序和 维护特定化验、仪器性能和维护的质量控制(QC)记录 历史。核心人员为调查人员进行分析，并培训学生和研究员在理论和 MS的实用方面MS Core是作为一个开放访问设施运行的，用户通常在其中准备 如果他们愿意的话，可以使用他们的样本和操作仪器。蛋白质组学样本被提交给核心 以供蛋白质组学工作人员在调查员和核心工作人员之间进行协商后进行分析 最合适的分析服务级别。人员负责样品处理的方方面面， 对提交进行蛋白质组学分析的样本进行分析和数据报告。行政人员监督 调查人员对仪器设施的使用情况，并编写关于使用情况的报告，供 管理核心。MSRC有14台质谱仪可供用户使用，除了专门的 用于二维差示凝胶电泳和凝胶成像的设备。BMSC的目标是：1)提供 高质量的GC/MS、串联LC/MS和MALDI/TOF质谱学服务 分子代谢物；2)为个体鉴定和鉴定提供蛋白质组学服务 蛋白质和更复杂的组织特定蛋白质组；(3)提供质谱学方面的分析专业知识 用于分析开发和验证；4)协助用户进行数据分析；5)在以下方面提供高级培训 生物医学质谱学和蛋白质组学。核心的目标是增强 研究人员预防、诊断或治疗人类消化系统疾病相关疾病的能力。

The Bioanalytical/Mass Spectrometry and Proteomic Core (BMSC) provides cost-effective, state-of-the-art instrumentation and expertise to investigators in the Vanderbilt Digestive Disease Research Center (VDDRC). This core is among the specialized service cores utilizing the technical personnel and instrument facilities located in the Vanderbilt Mass Spectrometry Research Center (MSRC). This core will be used for identification and quantitation of small molecule metabolites and identification and characterization of proteins. The core has been used extensively by 22 VDDRC investigators during the previous five years to assist users in developing analytical methods in experimental design, standard operating procedures, and maintain quality control (QC) records on particular assays, instrument performance, and maintenance history. Core personnel perform assays for investigators and train students and fellows in the theoretical and practical aspects of MS. The MS Core is run as an open-access facility in which users generally prepare their samples and operate the instruments if they so desire. Proteomics samples are submitted to the core for analysis by proteomics staff after consultations between the investigator and core staff have determined the most appropriate class of analytical service. Personnel handle all aspects of sample processing, analysis, and data reporting of samples submitted for proteomics analysis. Administrative staff monitor the use of the instrument facilities by investigators and prepare reports on utilization for use by the Administrative Core. The MSRC has 14 mass spectrometers available to users, in addition to specialized facilities for 2D-differential gel electrophoresis and gel imaging. The Aims of the BMSC are to: 1) provide high-quality GC/MS, tandem LC/MS, and MALDI/TOF mass Spectrometry services for analysis of small molecule metabolites; 2) provide proteomics services for identification and characterization of individual proteins and more complex tissue-specific proteomes; (3) provide analytical expertise in mass Spectrometry for assay development and validation; 4) assist users with data analysis; and 5) provide advanced training in biomedical mass Spectrometry and proteomics to students and fellows. The goal of the Core is to enhance investigator abilities to prevent, diagnose or treat human digestive disease-related disorders.