RETINAL CIRCUITS AND SYNAPSES FOR PRIMATE COLOR VISION

灵长类动物色觉的视网膜回路和突触

• 批准号: 8357626
• 负责人: DENNIS MICHAEL DACEY
• 金额: $ 15.66万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357626
• 关键词: AMPA Receptors; Cells; Code; Color; Color Visions; Complex; Contrast Sensitivity; Data; Funding; Glutamate Receptor; Goals; Grant; Human; In Vitro; Lateral Geniculate Body; Macaca; Modeling; Monkeys; N-Methyl-D-Aspartate Receptors; N-Methylaspartate; National Center for Research Resources; Photoreceptors; Preparation; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Retina; Retinal; Retinal Cone; Role; Series; Source; Stimulus; Synapses; Testing; United States National Institutes of Health; Vision; Visual Cortex; Work; cost; design; ganglion cell; neurotransmission; parvocellular; patch clamp; receptor; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Human trichromatic color vision arises from 'blue-yellow' and 'red-green' cone-driven channels. The blue-yellow channel compares the quantal catch of short- (S) with long- (L) and middle- (M) wavelength-sensitive photoreceptor types, and the red-green channel compares the quantal catch of L and M cones. Color vision begins in the complex retinal circuitry and in the projection to the parvocellular layers of the lateral geniculate nucleus (LGN) and, subsequently, to visual cortex. In the primate retina, two ganglion cell classes have been studied for their roles in blue-yellow and red-green color vision: the small bistratified and midget cell, respectively. However, several key questions remain unanswered about the roles of excitatory and inhibitory synaptic input in color-coding, as experimental approaches in the primate retina have been limited. For the proposed project, we will use our well-established in vitro retina preparation from the macaque monkey, an ideal model for understanding human vision, to determine the roles of excitatory and inhibitory neurotransmission in blue-yellow and red-green chromatic coding. To achieve these goals, small bistratified and midget ganglion cells will be targeted using loose-patch and whole-cell patch-clamp recording combined with pharmacological manipulation of excitatory and inhibitory receptors, conductance analysis, and short- (S), middle- (M), and long- (L) wavelength cone-selective stimuli to determine the roles of S-, L-, and M-cone-driven excitation and inhibition to a ganglion cell. A series of experiments have been designed to characterize the roles of ionotropic glutamate receptors (AMPA- and NMDA-type) in chromatic contrast sensitivity. Previous work has demonstrated complementary roles for AMPA and NMDA receptors in achromatic coding and our preliminary data suggests that these receptors are also important for color-coding. Direct testing of the roles of two types of inner retinal inhibition, feedforward and crossover, in color-coding will be performed.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 人类的三原色视觉来自“蓝-黄”和“红-绿”锥体驱动的通道。蓝-黄通道比较了短(S)和长(L)和中(M)波长敏感型感光器类型的量子捕获，红-绿色通道比较了L和M视锥的量子捕获。色觉始于复杂的视网膜回路，并投射到外侧膝状核(LGN)的小细胞层，随后投射到视觉皮质。在灵长类动物的视网膜中，人们研究了两类神经节细胞在蓝-黄和红-绿色视觉中的作用：分别是小的双层细胞和侏儒细胞。然而，关于兴奋性和抑制性突触输入在颜色编码中的作用，几个关键问题仍然没有得到回答，因为在灵长类视网膜中的实验方法一直受到限制。在拟议的项目中，我们将使用我们成熟的猕猴视网膜体外准备，这是理解人类视觉的理想模型，以确定兴奋性和抑制性神经传递在蓝-黄和红-绿颜色编码中的作用。为了实现这些目标，我们将利用松散膜片和全细胞膜片钳记录，结合兴奋性和抑制性受体的药理学操作，电导分析，以及短(S)、中(M)和长(L)波长的锥体选择性刺激，来确定S、L和M锥体驱动的兴奋和抑制对神经节细胞的作用。已经设计了一系列实验来表征离子型谷氨酸受体(AMPA和NMDA型)在颜色对比敏感度中的作用。以前的工作已经证明了AMPA和NMDA受体在非彩色编码中的互补作用，我们的初步数据表明，这些受体对颜色编码也很重要。将直接测试两种类型的视网膜内抑制在颜色编码中的作用，前馈和交叉。