PROGESTERONE RECEPTORS & ACTION IN PRIMATE OVARY

黄体酮受体

• 批准号: 8357733
• 负责人: RICHARD L STOUFFER
• 金额: $ 7.28万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357733
• 关键词: Binding Proteins; Cell physiology; Corticotropin-Releasing Hormone; Development; Enzymes; Fertility; Funding; Gene Expression; Genes; Graafian Follicles; Grant; Hormonal; Longevity; Luteinizing Hormone; Luteolysis; Menstrual cycle; Molecular; Monkeys; National Center for Research Resources; Ovarian; Ovary; Ovulation; Peptide Hydrolases; Periodicity; Pituitary Hormones; Primates; Principal Investigator; Process; Progesterone; Progesterone Receptors; Proteins; Research; Research Infrastructure; Resources; Source; Steroids; Structure; System; Testing; Tissues; United States National Institutes of Health; Woman; corpus luteum; cost; genome-wide; protein expression; receptor binding; receptor expression; research study; steroid hormone; urocortin

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The objective of this research is to understand the mechanisms whereby hormonal and local factors control the structure-function of the ovulatory follicle and corpus luteum in the ovary, and hence fertility, during the menstrual cycle in primates. Ongoing research is testing the hypothesis that the vital actions of the pituitary hormone, luteinizing hormone (LH) in promoting ovulation of the mature follicle and its subsequent conversion into the corpus luteum, and in controlling the functional lifespan of the corpus luteum, is either direct or indirect via local steroid action (e.g., via stimulation of progesterone [P] synthesis and P receptor expression). Because of similarities to ovarian function in women, monkeys are used in experiments where either LH and steroid hormones are depleted or steroids alone are depleted and replaced. The ovulatory follicle or corpus luteum is then removed to analyze molecular and cellular processes that are LH-dependent and either steroid/P-independent or -dependent. Gene and protein expression are analyzed for various processes, including: (1) protease enzymes that may be critical for tissue remodeling during ovulation, luteal development and luteal regression, and (2) local regulatory systems, e.g., the corticotropin-releasing hormone (CRH)/urocortins (UCN)-receptor-binding protein system. Current genome-wide analyses are identifying processes that are regulated by LH and/or steroid hormones in the follicle or corpus luteum. The activity of these processes is being manipulated, via gene knockdown or protein antagonist approaches, to elucidate their biologic relevance to primate ovarian function and cyclicity.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 本研究的目的是了解激素和局部因素在灵长类动物月经周期中控制卵巢排卵卵泡和黄体的结构功能，从而控制生育力的机制。正在进行的研究正在测试这样的假设：垂体激素、黄体生成素 (LH) 在促进成熟卵泡排卵及其随后转化为黄体以及控制黄体功能寿命方面的重要作用是直接或通过局部类固醇作用（例如，通过刺激孕酮 [P] 合成和 P 受体表达）间接发挥作用。由于与女性卵巢功能相似，猴子被用于实验，其中 LH 和类固醇激素被耗尽，或者单独类固醇被耗尽并被替换。然后取出排卵卵泡或黄体，以分析 LH 依赖性以及类固醇/P 独立或依赖性的分子和细胞过程。分析各种过程的基因和蛋白质表达，包括：（1）可能对排卵、黄体发育和黄体退化期间的组织重塑至关重要的蛋白酶，以及（2）局部调节系统，例如促肾上腺皮质激素释放激素（CRH）/尿皮质素（UCN）受体结合蛋白系统。目前的全基因组分析正在识别受卵泡或黄体中 LH 和/或类固醇激素调节的过程。通过基因敲低或蛋白质拮抗剂方法操纵这些过程的活性，以阐明它们与灵长类动物卵巢功能和周期性的生物学相关性。