MRI OF MARMOSETS

狨猴的 MRI

• 批准号: 8357701
• 负责人: MICHAEL DUFF DAVIS
• 金额: $ 6.66万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357701
• 关键词: Adolescent; Adult; Aging; Animal Model; Animals; Area; Atlases; Biochemical Process; Biomedical Research; Birth; Brain; Brain Diseases; Callithrix; Callithrix jacchus jacchus; Cell Nucleus; Classification; Complex; Data Set; Databases; Development; Funding; Grant; Histologic; Human; Image; Individual; Life; Life Cycle Stages; Longevity; Magnetic Resonance Imaging; Maps; Measurement; Mental disorders; Metabolic; Methods; Mission; Modeling; National Center for Research Resources; Neonatal; Neurons; Normal tissue morphology; Pattern; Phase; Positron-Emission Tomography; Primates; Principal Investigator; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Source; Structure; Time; United States National Institutes of Health; Validation; Weight; age related; aged; base; cost; gray matter; imaging modality; nervous system disorder; neuroimaging; senescence; white matter

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Progress in the study and treatment of human brain disease in development and aging can be greatly facilitated through the use of appropriate research animal models. This proposal seeks to build an extensive primate brain database of the common marmoset (Callithrix jacchus) that will define the evolving changes in anatomical structure, biochemical processes and neuronal activation patterns across the animal's entire life cycle. The marmoset brain is remarkably complex and is an archetype of human neurological and psychiatric disorders, yet has advantages over other old-world primate species such as its smaller size, is shorter-lived (6-12yrs), thereby having a more compressed rate of development and repopulates rapidly. Validation of this model first requires establishing the temporal course of normal tissue changes from birth through maturation and into senescence. Neuroimaging methods offer key advantages of being non-invasive and survivable, supporting an essentially unlimited number of sequential measurements over a prolonged period. Thus we will evaluate neonatal, juvenile, adult and aged marmoset groups in 4 specific aims by: [1] Describing the morphological development of global and regional brain structures by volumetric quantification of whole-brain, gray matter, white matter, gyri and sulci using T1-weighted anatomical magnetic resonance imaging (MRI). [2] Profiling white-matter area formation by employing multi-parametric T1- and T2- weighted MRI. [3] Characterizing gray-matter metabolic patterns in maturation and pruning phases using high-resolution 18F-FDG positron emission tomography (PET). [4] Defining the cytoarchitectonic microstructure of cortical layers and subcortical regions in immature, adult and aged marmoset brains by building a histologically-based stereotaxic atlas to include the taxonomical classification of individual features (e.g., nuclei, white matter tracts). This atlas will also be used to co-register the PET and MRI images for accurate mapping of topographical boundaries and substructures. Our overarching hypothesis is that the complex temporal profiles of age-related changes observed with multi-modality imaging in the human brain will be closely mirrored in the marmoset brain, but with shortened time constants reflecting its shorter life span. Fulfilling this mission will generate several comprehensive, descriptive developmental datasets and a translational neuroimaging toolkit that will be made broadly available to other investigators for application in a variety of biomedical research paradigms.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 通过使用适当的研究动物模型，可以大大促进人类大脑发育和衰老疾病的研究和治疗进展。该提案旨在建立一个广泛的灵长类动物脑数据库的普通绒猴（Callithrix jacchus），将定义在动物的整个生命周期中的解剖结构，生化过程和神经元激活模式的不断变化。绒猴的大脑非常复杂，是人类神经和精神疾病的原型，但与其他旧世界灵长类动物相比，绒猴的大脑具有优势，例如体积较小，寿命较短（6- 12岁），因此具有更压缩的发育速度和快速繁殖。该模型的验证首先需要建立从出生到成熟和衰老的正常组织变化的时间过程。神经影像学方法提供了非侵入性和可存活性的关键优势，支持在长时间内基本上无限数量的连续测量。因此，我们将通过以下4个特定目标评估新生、幼年、成年和老年绒猴组：[1]使用T1加权解剖磁共振成像（MRI）通过全脑、灰质、白色物质、脑回和脑沟的体积定量描述整体和局部脑结构的形态发育。[2]采用多参数T1和T2加权MRI描绘白质区形成。[3]使用高分辨率18F-FDG正电子发射断层扫描（PET）表征成熟和修剪阶段的灰质代谢模式。 [4]通过构建基于组织学的立体定位图谱来定义未成熟、成年和老年绒猴脑中皮质层和皮质下区域的细胞结构微结构，以包括个体特征的分类学分类（例如，核、白色物质束）。 该图集还将用于共配准PET和MRI图像，以准确绘制地形边界和子结构。 我们的总体假设是，在人类大脑中观察到的多模态成像与年龄相关的变化的复杂的时间曲线将密切反映在绒猴大脑中，但缩短的时间常数反映了其较短的寿命。 完成这一使命将产生几个全面的，描述性的发展数据集和翻译神经成像工具包，将广泛提供给其他研究人员在各种生物医学研究范式的应用。