DEVELOPMENT OF RESPIRATORY VIRUS INFECTION STRATEGIES FOR RHESUS MACAQUE AIRWAYS

恒河猴呼吸道呼吸道病毒感染策略的制定

• 批准号: 8357293
• 负责人: LISA M MILLER
• 金额: $ 2.52万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357293
• 关键词: Animal Model; Asthma; California; Cells; Child; Chronic; Clinical; Development; Etiology; Experimental Animal Model; Funding; Goals; Grant; Human; Human respiratory syncytial virus; Immune; Immune system; In Vitro; Infection; Lung; Macaca mulatta; Modeling; Monkeys; National Center for Research Resources; Physiological; Predisposition; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Respiratory Syncytial Virus Infections; Respiratory Tract Infections; Rhinovirus; Rodent Model; Role; Source; Symptoms; United States National Institutes of Health; Vaccines; Viral; airway inflammation; cost; in vivo; postnatal; prevent; respiratory infection virus; respiratory virus

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our primary objective is to understand the pathogenic role of respiratory virus infection in the etiology of asthma and exacerbation of chronic airways inflammation in humans. Our secondary objective is to develop effective and safe vaccine approaches to prevent respiratory syncytial virus infection in young children. We propose that basic immune and physiologic mechanisms for respiratory virus pathobiology in the lung can only be elucidated using an in vivo animal model of infection. Although rodent models have been developed for respiratory virus infection, this species does not effectively duplicate the postnatal development of both lung and immune systems in primates. Our goal is to develop an experimental animal model of human respiratory syncytial virus and human rhinovirus infection using the rhesus macaque monkey (Macaca mulatta). Towards this end, the first step is to optimize the susceptibility of rhesus monkeys to human respiratory virus infection. We hypothesize that the development of a respiratory infection model that demonstrates human clinical symptoms will require in vitro passaging of human viral isolates in rhesus cells to obtain robust viral replication in monkey airways.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 我们的主要目标是了解呼吸道病毒感染在人类哮喘病因和慢性气道炎症恶化中的致病作用。我们的次要目标是开发有效且安全的疫苗方法来预防幼儿呼吸道合胞病毒感染。我们认为，肺部呼吸道病毒病理学的基本免疫和生理机制只能使用体内感染动物模型来阐明。尽管已经开发出用于呼吸道病毒感染的啮齿动物模型，但该物种并不能有效地复制灵长类动物出生后肺和免疫系统的发育。我们的目标是利用恒河猴（Macaca mulatta）开发人类呼吸道合胞病毒和人类鼻病毒感染的实验动物模型。为此，第一步是优化恒河猴对人类呼吸道病毒感染的易感性。我们假设，开发展示人类临床症状的呼吸道感染模型将需要在恒河猴细胞中体外传代人类病毒分离株，以获得猴气道中强大的病毒复制。