DEVELOPMENT MOLECULARBIOMARKERS: MEASURE ENVIRONMENTALLY INDUCED IMMUNE RESPONSE

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 本研究的目的是建立一组免疫生物标志物，用于检测人类环境诱导的疾病，重点是过敏和哮喘的参数。 免疫生物标志物组将通过完成以下两个具体目标来开发：（1）开发白细胞分化标志物簇的高通量定量RT-PCR测定，以测量小生物样品中与过敏和哮喘相关的关键免疫细胞群，以及（2）开发高通量定量RT-PCR测定，PCR检测与过敏和哮喘相关的关键细胞因子标志物，以测量小生物样品中的免疫细胞功能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this study is to establish a panel of immune biomarkers that will be used to detect environmentally induced disease in humans, focusing on parameters of allergy and asthma. The panel of immune biomarkers will be developed by completion of the following two specific aims: (1) Develop a high-throughput quantitative RT-PCR assay for leukocyte cluster of differentiation markers to measure key immune cell populations associated with allergy and asthma in small biological samples and (2) Develop a high-throughput quantitative RT-PCR assay for key cytokine markers associated with allergy and asthma to measure immune cell function in small biological samples.