DEVELOPMENT MOLECULARBIOMARKERS: MEASURE ENVIRONMENTALLY INDUCED IMMUNE RESPONSE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这项研究的目的是建立一组免疫生物标记物，用于检测环境引起的人类疾病，重点是过敏和哮喘的参数。免疫生物标记物小组将通过完成以下两个具体目标来开发：(1)开发一种高通量的白细胞分化标记物的定量RT-PCR检测方法，以测量小生物样品中与过敏和哮喘相关的关键免疫细胞群；(2)开发一种高通量的与过敏和哮喘相关的关键细胞因子标记物的RT-PCR检测方法，以测量小生物样品中的免疫细胞功能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The objective of this study is to establish a panel of immune biomarkers that will be used to detect environmentally induced disease in humans, focusing on parameters of allergy and asthma. The panel of immune biomarkers will be developed by completion of the following two specific aims: (1) Develop a high-throughput quantitative RT-PCR assay for leukocyte cluster of differentiation markers to measure key immune cell populations associated with allergy and asthma in small biological samples and (2) Develop a high-throughput quantitative RT-PCR assay for key cytokine markers associated with allergy and asthma to measure immune cell function in small biological samples.