EXPANDING TOOLS FOR GENOME-WIDE ANALYSIS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心赠款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 两个独立的和生物学上无关的项目的描述是由该设备支持的，即具有增强的突触连接的线虫中的基因表达谱，以及在突触上激的E. coli细胞。这两项研究都包括使用DNA微阵列，这是一种最近普遍使用的设备。微阵列是一种强大的遗传工具，但微阵列数据的有效性并没有被普遍接受，在同行评审期刊上发表通常需要通过实时PCR获得所述数据。实时PCR循环仪的应用超出了本提案中描述的两个项目。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Descriptions of two separate and biologically unrelated projects are supported by this equipment, i.e. gene expression profiles in nematodes with enhanced synaptic connectivity, and gene expression profiles in osmotically upshocked E. coli cells. Both studies include the use of DNA microarrays, a device that has recently come into common use. Microarrays are a powerful genetic tool, but the validity of microarray data is not universally accepted and publication in peer-reviewed journals often requires of said data via real-time PCR. Application of the real-time PCR cycler extends beyond the two projects described in this proposal.