COMPOSITION ANALYSIS BY GC-MS OF THREE SAMPLES
通过 GC-MS 对三个样品进行成分分析
基本信息
- 批准号:8361851
- 负责人:
- 金额:$ 0.18万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-02-01 至 2012-01-31
- 项目状态:已结题
- 来源:
- 关键词:AcetylationBlood capillariesFundingGasesGlycosidesGrantGuanine + Cytosine CompositionInositolMethanolMethodsNational Center for Research ResourcesPrincipal InvestigatorReagentResearchResearch InfrastructureResourcesSamplingSeriesSilicon DioxideSourceTimeTubeUnited States National Institutes of Healthacetic anhydridecapillarycostpyridine
项目摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
Methods:
Glycosyl composition by GC-MS
The samples (~20 ¿g each) were placed in screw-cap tubes, 2 ¿L 1 mg/mL inositol was added as internal standard, and the samples were lyophilized. Methyl glycosides then were prepared from the dried samples by methanolysis with 3 M HCl in methanol at 100 ¿C for 2 h followed by re-N-acetylation with pyridine and acetic anhydride in methanol. The preceding methanolysis and re-N-acetylation steps were repeated two times. The samples then were per-O-trimethylsilylated (TMS) with a Tri-Sil reagent (Thermo Scientific) at 80 ¿C for 0.5 h. Analysis of the TMS methyl glycosides was performed on a Hewlett Packard Series II 5890 gas chromatograph equipped with a Supelco EC-1 fused silica capillary column (30m ¿ 0.25 mm ID) and interfaced to a Hewlett Packard 5970 MSD.
这个子项目是利用这些资源的众多研究子项目之一
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Parastoo Azadi其他文献
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{{ truncateString('Parastoo Azadi', 18)}}的其他基金
A National Glycoscience Resource - CCRC Service and Training
国家糖科学资源 - CCRC 服务和培训
- 批准号:
10025496 - 财政年份:2020
- 资助金额:
$ 0.18万 - 项目类别:
A National Glycoscience Resource - CCRC Service and Training
国家糖科学资源 - CCRC 服务和培训
- 批准号:
10265506 - 财政年份:2020
- 资助金额:
$ 0.18万 - 项目类别:
A National Glycoscience Resource - CCRC Service and Training
国家糖科学资源 - CCRC 服务和培训
- 批准号:
10707084 - 财政年份:2020
- 资助金额:
$ 0.18万 - 项目类别:
Glycan linkage and sequence plus determination of site of glycosylation by permethylation of glycopeptides and MSn analysis in a one pot experiment
一锅实验中的聚糖连接和序列以及通过糖肽全甲基化和 MSn 分析确定糖基化位点
- 批准号:
9337473 - 财政年份:2016
- 资助金额:
$ 0.18万 - 项目类别:
Glycan linkage and sequence plus determination of site of glycosylation by permethylation of glycopeptides and MSn analysis in a one pot experiment
一锅实验中的聚糖连接和序列以及通过糖肽全甲基化和 MSn 分析确定糖基化位点
- 批准号:
9166719 - 财政年份:2016
- 资助金额:
$ 0.18万 - 项目类别:
N-LINKED GLYCOSYLATION SITE MAPPING OF HIV-1 GP120
HIV-1 GP120 的 N 联糖基化位点定位
- 批准号:
8363095 - 财政年份:2011
- 资助金额:
$ 0.18万 - 项目类别:
MONOSACCHARIDE COMPOSITION ANALYSIS BY HPAEC
通过 HPAEC 进行单糖成分分析
- 批准号:
8363087 - 财政年份:2011
- 资助金额:
$ 0.18万 - 项目类别:
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