ANALYTICAL TECHNIQUES FOR STRUCTURAL ANALYSIS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 与会者将学习表征糖胺多聚糖(GAG)结构的基本技术，包括使用适当的酶将GAG多糖解聚为其二糖组分。二糖用离子交换层析分离，产物用紫外可见光谱检测，用2-氰基乙酰胺衍生化，用荧光检测法进行检测。在另一项实验中，糖胺多聚糖将被亚硝酸水解，并对产物进行分析。从糖胺多聚糖中提取的二糖和低聚糖的质谱学技术，以及硫酸盐和多糖染料结合分析也将被包括在内。基本的生化技术经验是参加的先决条件。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Participants will learn basic techniques for the structural characterization of glycosaminoglycans (GAGs), which will include depolymerization of the GAG polysaccharides to its disaccharide constituents using appropriate enzymes. The disaccharides will be separated by ion exchange chromatography and the products will be detected by U.V. The disaccharides will also be derivatized with 2-cyanoacetamide and detected with fluorescent detection. In a separate experiment, the glycosaminoglycans will be hydrolyzed by nitrous acid and the products will be analyzed. Mass spectrometry techniques for di- and oligosaccharides derived from glycosaminoglycans will also be covered, as well as sulfate and polysaccharide dye binding assays. Experience with basic biochemical techniques is a prerequisite for participation.