STRUCTURE OF E COLI RECEPTOR KINASES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 组氨酸激酶受体(HKR)是一种位于细胞膜上的蛋白质，其作用是感知环境刺激。为了将信号传递给细胞并触发对刺激的反应，它们与细胞内的伙伴蛋白一起工作，称为反应调节(RR)。HKRs和RRs形成了所谓的双组分系统(TCS)，这是原核生物中主要的信号转导机制，也存在于真菌和植物中。大多数HKRs有两个跨膜结构域，一个是周质感受器结构域，另一个是负责信号转导到RR的大细胞质结构域。在大肠杆菌中有23个已鉴定的HKR，但没有一个全长蛋白质的结构已知。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. E. coli histidine kinase receptors (HKR) are proteins located in the cell membrane and their role is to sense environmental stimuli. In order to transmit the signal to the cell and trigger a response to the stimulus they work together with their intracellular partner proteins, known as response regulators (RR). HKRs and RRs form the so-called Two Component Systems (TCS), which are the predominant signal transduction mechanisms in prokaryotic organisms, also present in fungi and plants. Most HKRs have two transmembrane domains ,a periplasmic sensor domain, and a large cytoplasmic domain which is responsible for the transduction of the signal to the RR. There are 23 identified HKRs in E. coli but a structure of no full length protein is known.