O-GLCNAC IN EMBRYONIC STEM CELLS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 SOX2和许多其他转录因子一样，都是O-GlcNacylated的。为了更好地理解多能性维持过程中的O-GlcN酰化，人们必须能够：1)无偏见地鉴定所涉及的低亲和力O-GlcN酰化核蛋白；2)为O-GlcN酰化指定位点特异性残基；3)理解O-GlcNAc信号来自什么细胞环境，因为许多核事件来自核外信号。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Sox2, as well as many other transcription factors, are known to be O-GlcNAcylated. In order to better understand O-GlcNAcylation during pluripotency maintenance, one must be able to i) identify the low abudnace O-GlcNAcylated nuclear proteins involved without bias, ii) assign site-specific residues for O-GlcNAcylation and iii) understand from what cellular context the O-GlcNAc signal came, since many nuclear events come from extranuclear signals.