TOWARD AN OPTIMIZED PROTEIN-PROTEIN CROSS-LINKING STRATEGY FOR PROTEIN COMPLEXES
蛋白质复合物的优化蛋白质-蛋白质交联策略
基本信息
- 批准号:8363770
- 负责人:
- 金额:$ 1.67万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-06-01 至 2012-05-31
- 项目状态:已结题
- 来源:
- 关键词:BioinformaticsChemicalsCross-Linking ReagentsDevelopmentEstersFlowersFundingGoalsGrantHydrolysisMapsMass Spectrum AnalysisMethodologyNational Center for Research ResourcesPeptidesPrincipal InvestigatorProteinsReactionReagentResearchResearch InfrastructureResolutionResourcesSourceStructureTechniquesUnited States National Institutes of Healthcostcrosslinkdesignmass analyzerprotein complextool
项目摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
With the advent of high mass accuracy/resolution mass analyzers suitable for the analysis of covalently modified peptides and proteins, chemical crosslinking has blossomed as a technique for mapping interacting domains and providing low resolution structures of protein complexes. The goal of this project is to extend the utility and scale of chemical crosslinking, which has generally been limited to the study of simple, binary protein interactions, to larger ensembles of cellular machinery. The largest barrier to applying structural crosslinking on this scale is the miniscule yield of intramolecular crosslinking resulting from reactions with traditional activated ester type crosslinking reagents. Reagents of this type are subject to hydrolysis, and as a result unmodified and "dead-end" modified peptides tend to predominate the reaction products of crosslinking reactions. This project encompasses: 1) the design and synthesis of new crosslinking reagents, 2) the development of methodology to enrich specifically crosslinked peptides and 3) the development of bioinformatic tools to assist in identifying crosslinked MS/MS spectra resulting from a large pool of interacting proteins.
这个子项目是许多利用资源的研究子项目之一
由NIH/NCRR资助的中心拨款提供。子项目的主要支持
而子项目的主要调查员可能是由其他来源提供的,
包括其它NIH来源。 列出的子项目总成本可能
代表子项目使用的中心基础设施的估计数量,
而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。
随着适用于共价修饰的肽和蛋白质的分析的高质量准确度/分辨率质量分析仪的出现,化学交联作为用于映射相互作用结构域和提供蛋白质复合物的低分辨率结构的技术已经蓬勃发展。 该项目的目标是将化学交联的实用性和规模扩展到更大的细胞机器集合,化学交联通常仅限于简单的二元蛋白质相互作用的研究。 在这种规模上应用结构交联的最大障碍是由与传统活化酯型交联试剂的反应产生的分子内交联的极小产率。 这种类型的试剂经受水解,并且因此未修饰的和“死端”修饰的肽倾向于在交联反应的反应产物中占主导地位。 该项目包括:1)新交联试剂的设计和合成,2)富集特异性交联肽的方法学的开发,以及3)生物信息学工具的开发,以帮助识别由大量相互作用蛋白质产生的交联MS/MS谱。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('ALMA L BURLINGAME', 18)}}的其他基金
UTILIZATION OF QSTARXL MASS SPECTROMETER, LC SYSTEM & ASSOCIATED SOFTWARE
QSTARXL 质谱仪、LC 系统的使用
- 批准号:
8363759 - 财政年份:2011
- 资助金额:
$ 1.67万 - 项目类别:
STRUCTURAL ANALYSIS OF SCORPION VENOM VAEJOVIS MEXICANUS SMITHI
墨西哥蝎毒VAEJOVIS MEXICANUS SMITHI的结构分析
- 批准号:
8363774 - 财政年份:2011
- 资助金额:
$ 1.67万 - 项目类别:
OPTIMIZATION OF ETD TECHNIQUE ON THE LTQ ORBITRAP
LTQ Orbitrap 上 ETD 技术的优化
- 批准号:
8363798 - 财政年份:2011
- 资助金额:
$ 1.67万 - 项目类别:
CHARACTERIZATION OF THE EARLY APOPTOTIC MITOCHONDRIAL PROTEOME
早期凋亡线粒体蛋白质组的表征
- 批准号:
8363803 - 财政年份:2011
- 资助金额:
$ 1.67万 - 项目类别:
DECIPHERING THE PHOSPHOPROTEOME OF T LYMPHOCYTES
破译 T 淋巴细胞的磷酸蛋白质组
- 批准号:
8363775 - 财政年份:2011
- 资助金额:
$ 1.67万 - 项目类别:
VISUALIZATION OF QUANTITATIVE BIOLOGICAL MASS SPECTROMETRY DATA
定量生物质谱数据的可视化
- 批准号:
8363629 - 财政年份:2011
- 资助金额:
$ 1.67万 - 项目类别:
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