STRUCTURAL ANALYSIS OF INFLUENZA VIRAL PROTEINS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 对于流感病毒如何感染宿主，人们寻求彻底的分子理解。为此，我们克隆并表达了来自历史、当代和大流行菌株的流感病毒蛋白。到目前为止，我们已经从广泛的病毒中结晶出血凝素(HA)和神经氨酸酶(NA)的可溶性形式，新的靶标正在酝酿中，包括2009-2010年的大流行毒株。这些结构的确定对设计有效的疫苗和抗病毒药物具有公共卫生意义，也将为流感外壳蛋白的快速进化提供有价值的见解。此外，我们还提纯了针对这些外壳蛋白的单抗。这些单抗/抗原相互作用的结构特征将提供有关抗体识别的重要信息，这将有助于设计下一代流感疫苗。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A thorough molecular understanding is sought for how influenza virus infects a host. To this end, we have cloned and expressed influenza viral proteins from historical, contemporary and pandemic stains. To date we have crystallized soluble forms of both hemagglutinin (HA) and neuraminidase (NA) from a broad range of viruses and new targets are in the pipeline, including the pandemic strains of 2009-2010. Determination of these structures has public health interest for designing effective vaccines and antivirals, also will provide valuable insight to the rapid evolution of influenza coat proteins. In addition, we have raised and purified monoclonal antibodies (mAbs) to these coat proteins. Structural characterization of these mAb/antigen interactions will provide important information about antibody recognition that will assist in designing the next generation of influenza vaccines.