X-RAY MICROFLUIDIC FLOW CYTOMETER FOR SINGLE CELL METAL ANALYSIS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 正在开发的这一仪器将首次能够方便地确定单个细胞的无机成分，从而提供对群体内成分分布的直接了解，并允许进行旨在将其他细胞属性与化学成分相关联的研究。微流控装置是在18ID光束线上组装的。它安装在两个微型工作台上：一个用于驱动细胞通过系统的盲文，另一个用于微流体装置本身。微流控的速度被固定为每滴设置一个细胞。在第一次实验中，我们对仪器的不同参数进行了优化。(1)优化提供最佳荧光信号的液体的体积；(2)改变细胞大小，从较大的单个细胞大小开始，以降低细胞大小。根据第一个结果，我们现在正在修改或设计到优化的条件。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This instrument under development will allow, for the first time, facile determination of the inorganic composition of individual cells, thereby providing direct insight into the distribution of compositions within the population, and permitting studies aimed at correlating other cellular properties with chemical composition. The micro-fluidic device was assembled at the 18ID beamline. It was mounted on two micro stages: one for the Braille that drives the cells through the system and one for the micro fluidic device itself. The rate of the micro-fluidic was fixed to set one cell per drop. During the first experiment we work on the optimization of the different parameters of the instrument. (1) optimize the volume of liquid that will give the best possible fluorescence signal; (2) vary the cell size, starting with larger single cell size to lower cell size. Based on the first results we are now modifying or device to optimized conditions.