X-RAY MICROFLUIDIC FLOW CYTOMETER FOR SINGLE CELL METAL ANALYSIS

用于单细胞金属分析的 X 射线微流控流式细胞仪

• 批准号: 8361313
• 负责人: ANIRUDDHA DEB
• 金额: $ 1.77万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361313
• 关键词: Biophysics; Cell Size; Cells; Chemicals; Development; Devices; Drops; Fluorescence; Funding; Grant; Individual; Liquid substance; Metals; Microfluidics; National Center for Research Resources; Population; Principal Investigator; Property; Research; Research Infrastructure; Resources; Roentgen Rays; Signal Transduction; Source; Staging; System; Time; United States National Institutes of Health; Work; base; beamline; braille; cost; insight; instrument; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This instrument under development will allow, for the first time, facile determination of the inorganic composition of individual cells, thereby providing direct insight into the distribution of compositions within the population, and permitting studies aimed at correlating other cellular properties with chemical composition. The micro-fluidic device was assembled at the 18ID beamline. It was mounted on two micro stages: one for the Braille that drives the cells through the system and one for the micro fluidic device itself. The rate of the micro-fluidic was fixed to set one cell per drop. During the first experiment we work on the optimization of the different parameters of the instrument. (1) optimize the volume of liquid that will give the best possible fluorescence signal; (2) vary the cell size, starting with larger single cell size to lower cell size. Based on the first results we are now modifying or device to optimized conditions.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 正在开发的这一仪器将首次允许对单个细胞的无机组成进行简便的测定，从而提供对群体内组成分布的直接洞察，并允许旨在将其他细胞特性与化学组成相关联的研究。在18 ID光束线处组装微流体装置。它被安装在两个微型平台上：一个用于驱动细胞通过系统的盲文，另一个用于微流体设备本身。 固定微流体的速率以设定每滴一个细胞。 在第一次实验中，我们对仪器的不同参数进行了优化。(1)优化将给出最佳可能的荧光信号的液体体积;（2）改变细胞大小，从较大的单细胞大小开始到较低的细胞大小。基于第一个结果，我们现在正在修改或设备优化条件。