ESR STUDY OF BACTERIAL CYTOCHROME C

细菌细胞色素C的ESR研究

• 批准号: 8364045
• 负责人: BORIS G DZIKOVSKI
• 金额: $ 0.27万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364045
• 关键词: Chemistry; Funding; Grant; Helium; Heme; Investigation; Liquid substance; National Center for Research Resources; Principal Investigator; Relaxation; Research; Research Infrastructure; Resources; Series; Source; Technology; Time; United States National Institutes of Health; Universities; Variant; cofactor; cost; cryostat; cytochrome c; instrumentation; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Bren group from the University of Rochester's Department of Chemistry utilized the X-Band EPR instrumentation at ACERT to study a series of bacterial cytochrome c (cyt c) variants. These studies were part of an on-going investigation of the functional implications of out-of-plane distortions of the heme cofactor. The continuous-flow, liquid helium cryostat available at ACERT is essential for these experiments because the EPR spectra of cyts c are typically not observed above 30 K due to a relatively short spin-lattice relaxation time.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 来自罗切斯特大学化学系的Bren小组利用ACERT的X波段EPR仪器研究了一系列细菌细胞色素c（cyt c）变体。这些研究是一个正在进行的调查的功能影响的血红素辅因子的平面外扭曲的一部分。ACERT提供的连续流液氦低温恒温器对于这些实验是必不可少的，因为由于相对较短的自旋-晶格弛豫时间，在30 K以上通常观察不到细胞c的EPR谱。