NMR Metabolomics Core/SNYDER

NMR代谢组学核心将负责生成和分析生物体液和组织提取物的代谢组学图谱。该核心将通过以一致和连贯的格式提供标准化分析和数据，并提供在研究设计和其他表型指标和数据流背景下解释结果的专业知识，支持RTI RCMRC。RTI内部的研究 代谢组学计划包括样品分析（例如，尿、血清、血清提取物、血浆、肝、肾、脑、子宫、睾丸），以提供机理见解并开发预测不良健康结果的生物标志物。NMR代谢组学核心还具有评估来自人类受试者研究的样本的经验， 对治疗和疾病终点的反应。NMR代谢组学核心是由我们的合作伙伴从大卫H。默多克研究所，拥有最先进的700 MHz和950 MHz NMR光谱仪。三位国际公认的代谢组学科学家（Jeremy Nicholson博士，Elaine Holmes博士和Ian Wilson博士）将通过咨询和合作支持RTI RCMRC。这一核心 将确保采用最具成本效益的方法，对适当规模的批次进行样本分析，以降低成本。质量控制特征将包括使用内部标准品、预先确定重复测量方法的重现性、在中心活动的所有方面使用单一样品代码以及适当的稽查。核心成员将与RTI RCMRC管理团队合作，根据数据共享计划和要求以及EC，制定一致的格式和可接受的格式，以将原始数据或处理后的数据文件存款数据存储库和协调中心。NMR Core将开发和采用EC认为合适的新方法。国家监测和报告核心小组成员将与宣传和外联核心小组合作，编制在会议上分发的材料，并提供网上培训和实习培训。NMR核心将通过代谢物鉴定支持质谱核心。

The NMR Metabolomics Core will be responsible for the generation and analysis of metabolomic profiles of biological fluids and tissue extracts. This Core will support the RTI RCMRC by providing standardized analysis and data in a consistent and coherent format, and providing expertise for interpreting results in the context of the study design and other phenotypic measures and data streams. Studies within the RTI Metabolomics Program have included the analysis of samples (e.g., urine, serum, extracts of serum, plasma, liver, kidney, brain, uterus, testes) from dose-response studies in rodent models to provide mechanistic insights and to develop biomarkers that are predictive of adverse health outcomes. The NMR Metabolomics Core also has experience evaluating samples from human subject research aimed at examining the responsivity to treatment and disease endpoints. The NMR Metabolomics Core is supported by our partners from the David H. Murdock Research Institute, which houses state-of-the-art 700 MHz and 950 MHz NMR spectrometers. Three internationally recognized metabolomics scientists (Dr. Jeremy Nicholson, Dr. Elaine Holmes, and Dr. Ian Wilson) will support the RTI RCMRC though consultation and collaboration. This core will ensure that the most cost-effective approach is used by conducting analysis of samples in appropriate size batches to reduce costs. Quality control features will include the use of internal standards, prior determination of reproducibility of methods with repeat measures, using single sample code throughout all aspects of center activities, and appropriate audits. The Core members will work with the RTI RCMRC Management Team to develop consistent formats and acceptable formats to deposit raw data or processed data files to the Data Repository and Coordinating Center according to the data sharing plan and requirements and the EC. The NMR Core will develop and adopt new methods as deemed appropriate by the EC. Members of the NMR Core will work with the Promotion and Outreach Core to develop materials for dissemination at meetings, and to provide web-based and internship training. The NMR Core will support the Mass Spectrometry Cores through metabolite identification.