Macromolecular Crystallography at the National Synchrotron Light Source

国家同步加速器光源下的大分子晶体学

• 批准号: 8294541
• 负责人: ROBERT M SWEET
• 金额: $ 250.05万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-10-02 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8294541
• 关键词: Biological Preservation; Collaborations; Collimator; Computer software; Crystallography; Databases; Deposition; Development; Goals; Grant; Imagery; Light; Mails; Measurement; Methods; Proteins; Publications; Recruitment Activity; Research; Resources; Robotics; Scientist; Services; Source; Specimen; Specimen Handling; Synchrotrons; Technology; Training; base; beamline; cryogenics; detector; improved; operation; programs; research and development

The Macromolecular Crystallography Research Resource (PXRR) at the National Synchrotron Light Source (NSLS) operates six beamlines that are employed for research by about 160 outside research groups and half a dozen Brookhaven Lab groups. These scientists produce over 200 research publications in a typical year, and over 300 Protein Data Bank depositions in the last year. The PXRR have pioneered an active Mail-in program that involves active collaborations with about 15 outside research groups, and short collaborations or service projects with many more. This program has interacted with 90 groups in the last several years, and there have been about 25 PDB depositions and 15 publications from this program in each of the last four years. The PXRR performs research and development on apparatus, methods, and software to improve the technology for synchrotron-based macromolecular crystallography. Special emphasis during the coming cycle will be on development of detectors that will match the power and collimation of modern x-ray sources, use of micron-sized crystals, development of robotic cryogenic specimen handling, and simultaneous measurement of UV, Visible, or Raman IR spectra with x-ray diffraction. The PXRR staff will execute numerous small research programs, among other things to improve visualization of tiny specimens and improve cryogenic preservation of specimens. A long-term goal for the PXRR is to create a resource for macromolecular crystallography at NSLS-II, the new synchrotron light source that is being created at Brookhaven Lab during the next five to seven years. The objective will be to have several macromolecular crystallography beamlines in operation when NSLS-II begins operation. This project will involve direct cooperation with NSLS-II staff to develop a complete plan for such facilities, and then to begin to achieve that plan.

国家同步加速器光源（NSLS）的高分子晶体学研究资源（PXRR）运行着六条光束线，这些光束线被大约160个外部研究小组和六个布鲁克海文实验室小组用于研究。这些科学家在一年中发表了200多篇研究论文，去年发表了300多篇蛋白质数据库。PXRR开创了一个积极的邮寄计划，涉及与大约15个外部研究小组的积极合作，以及与更多的短期合作或服务项目。在过去的几年里，该方案与90个团体进行了互动，在过去的四年里，每年都有大约25份PDB证词和15份出版物来自该方案。 PXRR对设备，方法和软件进行研究和开发，以改进基于同步加速器的大分子晶体学技术。下一个周期的特别重点将是开发与现代X射线源的功率和准直相匹配的探测器，使用微米级晶体，开发机器人低温样品处理，以及用X射线衍射同时测量紫外、可见或拉曼红外光谱。PXRR的工作人员将执行许多小型研究项目，其中包括改善微小标本的可视化和改善标本的低温保存。 PXRR的一个长期目标是在NSLS-II上为大分子晶体学创造一个资源，NSLS-II是布鲁克海文实验室在未来五到七年内创建的新同步加速器光源。目标是在NSLS-II开始运行时有几条大分子晶体学光束线运行。该项目将涉及与NSLS-II工作人员的直接合作，为这些设施制定一个完整的计划，然后开始实现该计划。