Development Of Flow Cytometric Technologies For Immune Assays

免疫测定流式细胞术技术的发展

• 批准号: 8745614
• 负责人: Mario Roederer
• 金额: $ 116.85万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8745614
• 关键词: Algorithms; Biological Assay; Cells; Complex; Data; Data Analyses; Development; Flow Cytometry; Fluorescent Probes; Foundations; Immune; Immune response; Immunology; Laboratories; Lymphocyte; Lymphocyte Function; Measurement; Population; Techniques; Technology; Vaccinated; Vaccines; Virus; novel; pathogen; research study; response; tool

Flow Cytometry is the primary tool in most immunology laboratories for the detailed characterization of lymphocytes and lymphocyte function. The ability to resolve highly complex populations of cells, interrogate the cells one-by-one, make multiple measurements on each cell, and then separate the cells viably for further study make this technology unique and indispensable. Our laboratory has long been involved in pushing this technology forward; we have perhaps the most sophisticated flow cytometer in the world, currently capable of making 20 different measurements on each cell. This platform has proven invaluable in dissecting immune responses to vaccines and viruses. In addition, we are developing novel algorithms for the automated analysis of the highly complex data that results from these experiments.

流式细胞术是大多数免疫学实验室详细描述淋巴细胞和淋巴细胞功能的主要工具。能够分解高度复杂的细胞群体，逐个询问细胞，对每个细胞进行多次测量，然后生动地分离细胞以供进一步研究，这使得这项技术独一无二，不可或缺。我们的实验室长期以来一直致力于推动这项技术的发展；我们可能拥有世界上最复杂的流式细胞仪，目前能够对每个细胞进行20种不同的测量。事实证明，该平台在剖析疫苗和病毒的免疫反应方面具有无价的价值。此外，我们正在开发新的算法，用于自动分析这些实验产生的高度复杂的数据。