T Cell Dynamics In SIV-infected Macaques

感染 SIV 的猕猴中的 T 细胞动力学

• 批准号: 7592390
• 负责人: Mario Roederer
• 金额: $ 45.97万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7592390
• 关键词: Acute; Animals; Blood; CD8-Positive T-Lymphocytes; Cells; Clinical; Color; Disease; Disease Progression; Frequencies; Goals; HIV; Immune System Part; Immune response; Immune system; Infection; Lymphoid; Macaca; Organ; Outcome; Peripheral; Phase; Publishing; SIV; Site; Staging; T-Lymphocyte; T-Lymphocyte Subsets; Technology; Therapeutic; Treatment Protocols; Vaccines; Virus; Work; functional status; mucosal site; trafficking

While much work has been published on the dynamics of T cells-changes in representation of CD4 and CD8 cells-during HIV disease progression and treatment (HAART), much less is known about the changes in the functional subsets of T cells. Indeed, virtually nothing is known about the changes in the mucosal T cells-those cells residing in the gut or other peripheral sites (but can traffic via the blood and are found at low frequencies there). We are investigating the interrelationship of the mucosal and central immune systems, using our unique 17-color flow cytometric technology to derive as much information as possible from each animal. We are evaluating the functional status of the dozens of unique T cell subsets in blood, lymphoid organs, and mucosal sites during SIV disease and subsequent therapeutic regimens. We are also comparing these parameters in animals that have undergone vaccine regimens, to understand whether or not the vaccines have altered the specific immune response to the virus and the impact of such changes on clinical outcome.

虽然在HIV疾病进展和治疗（HAART）过程中，关于T细胞的动力学（CD4和CD8细胞的变化）的研究已经发表了很多，但对T细胞功能亚群的变化所知甚少。实际上，我们对粘膜T细胞的变化一无所知——这些细胞存在于肠道或其他外周部位（但可以通过血液运输，在那里发现的频率很低）。我们正在研究粘膜和中枢免疫系统的相互关系，使用我们独特的17色流式细胞术技术从每只动物身上获得尽可能多的信息。我们正在评估几十种独特的T细胞亚群在SIV疾病和随后的治疗方案中在血液、淋巴器官和粘膜部位的功能状态。我们还在接受疫苗治疗的动物中比较这些参数，以了解疫苗是否改变了对病毒的特异性免疫反应，以及这种变化对临床结果的影响。