Development of Aersolized Vaccines

我们已经证明，雾化重组腺病毒在运送到恒河猴肺内时具有高度的免疫原性。具体地说，这种免疫在肺内产生非常高水平的持续的CD4和CD8T细胞反应，尽管在身体的其他部位产生非常低的水平。有趣的是，我们还发现在整个身体和不同的粘膜部位都能产生非常好的抗体反应。因此，这条途径对于产生黏膜免疫反应可能是非常重要的，这是预防艾滋病毒等挑战所必需的。但可以肯定的是，高肺细胞反应是预防结核病或流感的理想选择；我们正在用载体免疫这些病原体，并测试是否有保护作用。

We have demonstrated that aerosolized recombinant adenovirus is highly immunogenic when delivered to the lung of rhesus macaques. Specifically, this immunization generates very high levels of persistent CD4 and CD8 T cell responses in the lung, although very low levels throughout the rest of the body. Interestingly, we also find very good generation of antibody responses, both throughout the body and at various mucosal sites. Thus, this route may be very important for the generation of mucosal immune responses needed to protect against challenges such as HIV. But certainly, the high lung cellular responses are ideal for the possible protection against tuberculosis or influenza; we are in the process of immunizing with vectors against these pathogens and testing whether or not protection is seen.