Development of Aersolized Vaccines

雾化疫苗的开发

• 批准号: 7592437
• 负责人: Mario Roederer
• 金额: $ 45.97万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7592437
• 关键词: Address; Adenoviruses; Antibodies; Antibody Formation; Antigens; Area; CD8B1 gene; Depth; Development; Generations; Goals; HIV; Immune response; Immunization; Influenza; Lung; Macaca mulatta; Mucosal Immune Responses; Process; Recombinants; Rest; Route; Site; Surface; T-Lymphocyte; Testing; Tuberculosis; Vaccines; aerosolized; immunogenic; mucosal site; novel strategies; pathogen; response; vector

We have demonstrated that aerosolized recombinant adenovirus is highly immunogenic when delivered to the lung of rhesus macaques. Specifically, this immunization generates very high levels of persistent CD4 and CD8 T cell responses in the lung, although very low levels throughout the rest of the body. Interestingly, we also find very good generation of antibody responses, both throughout the body and at various mucosal sites. Thus, this route may be very important for the generation of mucosal immune responses needed to protect against challenges such as HIV. But certainly, the high lung cellular responses are ideal for the possible protection against tuberculosis or influenza; we are in the process of immunizing with vectors against these pathogens and testing whether or not protection is seen.

我们已经证明，雾化的重组腺病毒在递送至恒河猴的肺部时具有高度免疫原性。 具体来说，这种免疫在肺部产生非常高水平的持续性 CD4 和 CD8 T 细胞反应，但在身体其他部位的水平非常低。 有趣的是，我们还发现在整个身体和各个粘膜部位都产生了非常好的抗体反应。 因此，这条途径对于产生抵御艾滋病毒等挑战所需的粘膜免疫反应可能非常重要。 但可以肯定的是，高肺细胞反应是预防结核病或流感的理想选择。我们正在使用针对这些病原体的载体进行免疫，并测试是否能看到保护作用。