Development of Aersolized Vaccines

雾化疫苗的开发

• 批准号: 7592437
• 负责人: Mario Roederer
• 金额: $ 45.97万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7592437
• 关键词: Address; Adenoviruses; Antibodies; Antibody Formation; Antigens; Area; CD8B1 gene; Depth; Development; Generations; Goals; HIV; Immune response; Immunization; Influenza; Lung; Macaca mulatta; Mucosal Immune Responses; Process; Recombinants; Rest; Route; Site; Surface; T-Lymphocyte; Testing; Tuberculosis; Vaccines; aerosolized; immunogenic; mucosal site; novel strategies; pathogen; response; vector

We have demonstrated that aerosolized recombinant adenovirus is highly immunogenic when delivered to the lung of rhesus macaques. Specifically, this immunization generates very high levels of persistent CD4 and CD8 T cell responses in the lung, although very low levels throughout the rest of the body. Interestingly, we also find very good generation of antibody responses, both throughout the body and at various mucosal sites. Thus, this route may be very important for the generation of mucosal immune responses needed to protect against challenges such as HIV. But certainly, the high lung cellular responses are ideal for the possible protection against tuberculosis or influenza; we are in the process of immunizing with vectors against these pathogens and testing whether or not protection is seen.

我们已经证明，雾化重组腺病毒是高度免疫原性时，交付给恒河猴肺。 具体地说，这种免疫在肺中产生非常高水平的持续性CD 4和CD 8 T细胞应答，尽管在身体的其余部分中水平非常低。 有趣的是，我们还发现在整个身体和各种粘膜部位都产生了非常好的抗体应答。 因此，这种途径对于产生保护免受HIV等挑战所需的粘膜免疫应答可能非常重要。 但可以肯定的是，高肺细胞反应是理想的可能保护对结核病或流感;我们正在与载体免疫这些病原体和测试是否保护被视为。