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Non-Human Primate Core

非人类灵长类核心

基本信息

批准号：
9161791
负责人：
Mario Roederer
金额：
$ 68.97万
依托单位：
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9161791
关键词：
Adjuvant Animal Care and Use Committees Animals Basic Science Biological Assay Cell Separation Clinical Collaborations Consult Contracts Cryopreservation Data Data Analyses Ensure Evaluation Flow Cytometry Generations Human IACUC Institutes International Laboratories Laboratory Animal Medicine Lymphocyte Maintenance Memory Methods Modeling Oral Pathogenesis Practice Guidelines Preclinical Testing Procedures Protocols documentation Qualifying Reproducibility Research Personnel Research Support SIV Schedule Site Specimen Specimen Handling Technology Testing Tissues Training Translational Research Translations Vaccination Vaccine Research Vaccines Virus Diseases Writing design good laboratory practice immune function immunogenicity mucosal site nonhuman primate operation preclinical study research study response sample collection trafficking vector

项目摘要

The Vaccine Research Center Nonhuman primate Immunogenicity Core supports the basic research laboratories of the VRC that are performing vaccination experiments with nonhuman primates. The Core has developed rigorous protocols for the isolation of cells, cryopreservation of cells, isolation of mucosal specimens, assays to detect immune function, and assays to determine lymphocyte representation in tissues. The NIC will qualify and validate these assays in order to provide the most rigorous support for basic research laboratories by ensuring consistency, sensitivity, and reproducibility. The Core has expertise in all aspects of NHP experiments, and provides support to investigators at multiple levels: design and implementation of experiments, writing and presenting the animal protocols to the Institutional Animal Care and Use Committee, scheduling of experiments, interfacing with the Laboratory of Animal Medicine and animal contract facilities, processing of specimens, data analysis. The Core also maintains a cryorepository of specimens, and participates in international training and collaborations to ensure translation of Core assays to other sites.

疫苗研究中心非人灵长类动物免疫原性核心支持VRC的基础研究实验室，这些实验室正在用非人灵长类动物进行疫苗接种实验。该中心已制定了严格的方案，用于分离细胞、冷冻保存细胞、分离粘膜标本、检测免疫功能的试验以及确定组织中淋巴细胞代表性的试验。 NIC将鉴定和验证这些检测试剂盒，以确保一致性、灵敏度和重现性，从而为基础研究实验室提供最严格的支持。核心在NHP实验的各个方面都有专业知识，并在多个层面上为研究人员提供支持：实验的设计和实施，编写和向机构动物护理和使用委员会提交动物方案，安排实验，与动物医学实验室和动物合同设施接口，标本处理，数据分析。该中心还维护一个标本冷冻库，并参与国际培训和合作，以确保将核心检测转化到其他地点。