Identification of HIV-specific B cells in blood

血液中 HIV 特异性 B 细胞的鉴定

• 批准号: 7592417
• 负责人: Mario Roederer
• 金额: $ 22.98万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7592417
• 关键词: Antibodies; Antigens; B-Lymphocytes; Binding; Blood; Cells; Flow Cytometry; Goals; HIV; HIV Infections; HIV envelope protein; Immune system; In Vitro; Maps; Monoclonal Antibodies; Mutate; Numbers; Protein Region; Proteins; Research; Techniques; Treatment Protocols; Vaccination; Vaccines; Viral Proteins; inhibiting antibody; interest; mucosal site; pathogen; response

We are using sophisticated flow cytometric techniques to characterize, in detail, the B cell responses to HIV infection and to HIV vaccination. Our goal is to identify the antigen-specific B cells, isolate them, and then map the responses of the B cells to the viral proteins. Specifically, we use fluorescently-conjugated antigens (for example, the HIV envelope protein), to identify cells which bind those proteins. By using mutated forms of the antigens, we can selectively isolate cells which bind only certain regions of the protein of interest. We hope to use this to identify antibodies (and B cells producing them) that selectively bind important regions of the HIV envelope, such as the CD4-binding domain.

我们正在使用先进的流式细胞仪技术来详细描述B细胞对艾滋病毒感染和对艾滋病毒疫苗接种的反应。我们的目标是识别抗原特异的B细胞，分离它们，然后绘制B细胞对病毒蛋白的反应图。具体地说，我们使用荧光标记的抗原(例如，HIV包膜蛋白)来识别与这些蛋白结合的细胞。通过使用抗原的突变形式，我们可以选择性地分离仅结合感兴趣蛋白质的特定区域的细胞。我们希望利用这一点来识别抗体(以及产生抗体的B细胞)，这些抗体选择性地结合艾滋病毒包膜的重要区域，如CD4结合域。