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Functional Genomics/Genomic Sequencing

功能基因组学/基因组测序

基本信息

批准号：
8450537
负责人：
MICHAEL A FRIEDMAN
金额：
$ 25.54万
依托单位：
BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-08-01 至 2017-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8450537
关键词：
Area Binding Biochemical Pathway Biological Biological Assay Biological Markers Cancer Biology Cancer Center Cancer Center Support Grant Cancer Gene Mutation Cell Proliferation Cell physiology Cells Classification Clinical Core Facility Coupled CpG Islands Custom DNA DNA Methylation DNA Microarray Chip DNA Sequence DNA Sequencing Facility DNA biosynthesis Dedications Development Developmental Process Diagnosis Diagnostic Disease Drug Targeting Elements Enzymes Epigenetic Process Eukaryota Evaluation Fingerprint Formalin Functional RNA Functional disorder Gene Chips Gene Expression Gene Expression Profile Gene Expression Profiling Gene Expression Regulation Gene Silencing Genes Genetic Recombination Genetic Transcription Genetic Variation Genome Genomics Genotype Goals Human Human Genome Individual Differences Instruction Investigation Investments Lead Link Malignant Neoplasms Maps Measurement Methylation Methyltransferase MicroRNAs Microfluidics Molecular Profiling Monitor Nucleotides Organism Paraffin Embedding Pathogenesis Pathologic Processes Pathway interactions Pattern Pharmaceutical Preparations Physiological Processes Proteins Publications RNA RNA Interference Relative (related person)Research Research Personnel Resolution Resource Sharing Role Safety Sampling Screening for cancer Services Signal Pathway Single Nucleotide Polymorphism Site Small RNA System Systems Analysis Technology Toxic effect Training Transcript Treatment Efficacy United States National Institutes of Health Validation anticancer research base cancer diagnosis cancer genetics cancer genomics cancer risk cancer therapy carcinogenesis epigenomics experience fighting functional genomics genome sequencing genome wide association study genome-wide histone modification human disease improved innovation instrument instrumentation metaplastic cell transformation molecular phenotype new therapeutic target next generation next generation sequencing novel novel therapeutics programs prospective rapid detection receptor response technology/technique therapeutic target tool transcriptome sequencing transcriptomics

项目摘要

PROJECT SUMMARY (See instructions); The overall goal of the Functional Genomics/Genomic Sequencing Core (FGC) is to provide state-of-the-art instruments, and expert scientific and technical advice in cancer genomics to COHCCC investigators. The FGC is equipped with major genomics instrumentation such as Affymetrix GeneChip¿ Analysis System, Agilent scanner/microarray system, Roche NimbleGen MS200 microarray scanner/system, lllumina HiScanSQ, HiSeq2000, GA llx and Roche 454 FLX. The core also has a next-generation ABI Taqman Realtime PCR system ViiA 7 for microarray validation. FGC provides comprehensive genomic support including transcriptomic and mlRNA/smRNA profiling by,microarrays and RNA-Seq/smRNA-Seq, ChlP-Chip/ChlPSeq, DNA methylation, DNA-Seq including whole genome and target genome sequencing, microarray genome-wide and custom genotyping, SNP/CNV, aCGH, RNAi and qRT-PCR. The FGC has recently set up numerous new genomic technologies and assays including microarray-coupled genome-wide gene expression profiling using difficult clinical formalin-fixed paraffin-embedded (FFPE) RNA samples, smRNASeq and RNA-Seq using FFPE-derived samples, microfluidic chip- and microarray-coupled single-cell genome-wide gene expression profiling. In addition, the FGC has implemented NimbleGen array-based comprehensive high-throughput arrays for relative methylation (CHARM), Affymetrix DMET and genomewide human SNP 6.0 array genotyping, and lllumina Infinium HumanMethylation450 BeadChip that interrogates more than 450,000 methylation sites across the whole human genome at single-nucleotide resolution. The FGC has supported numerous NIH/NCI projects resulting in high impact publications. In summary, the advanced genomic tools in the FGC allow COHCCC investigators to: 1) identify cancer gene mutations at high-throughput rates; 2) map cancer genomic, transcriptomic and epigenomic fingerprints to identify genomic biomarkers for cancer early detection and diagnosis; 3) identify novel therapeutic targets against cancers; and 4) predict responses to therapy.

项目摘要（参见说明）；功能基因组学/基因组测序核心 (FGC) 的总体目标是为 COHCCC 研究人员提供最先进的仪器以及癌症基因组学方面的专家科学和技术建议。 FGC 配备了主要的基因组仪器，如 Affymetrix GeneChip¿ 分析系统、Agilent 扫描仪/微阵列系统、Roche NimbleGen MS200 微阵列扫描仪/系统、lllumina HiScanSQ、HiSeq2000、GA llx 和 Roche 454 FLX。该核心还具有用于微阵列验证的下一代 ABI Taqman 实时 PCR 系统 ViiA 7。 FGC 提供全面的基因组支持，包括通过微阵列和 RNA-Seq/smRNA-Seq、ChlP-Chip/ChlPSeq、DNA 甲基化、DNA-Seq（包括全基因组和目标基因组测序）、微阵列全基因组和定制基因分型、SNP/CNV、aCGH、RNAi 和 qRT-PCR 进行转录组和 mlRNA/smRNA 分析。 FGC 最近建立了许多新的基因组技术和检测方法，包括使用困难的临床福尔马林固定石蜡包埋 (FFPE) RNA 样品进行微阵列耦合全基因组基因表达谱分析、使用 FFPE 衍生样品进行 smRNASeq 和 RNA-Seq、微流控芯片和微阵列耦合单细胞全基因组基因表达谱分析。此外，FGC 还实施了基于 NimbleGen 阵列的综合高通量阵列，用于相对甲基化 (CHARM)、Affymetrix DMET 和全基因组人类 SNP 6.0 阵列基因分型，以及 lllumina Infinium HumanMmethylation450 BeadChip，以单核苷酸分辨率询问整个人类基因组中超过 450,000 个甲基化位点。 FGC 支持众多 NIH/NCI 项目，并发表了高影响力的出版物。总之，FGC 中的先进基因组工具使 COHCCC 研究人员能够：1）以高通量率识别癌症基因突变； 2）绘制癌症基因组、转录组和表观基因组指纹图谱，以确定用于癌症早期检测和诊断的基因组生物标志物； 3）确定新的癌症治疗靶点； 4) 预测治疗反应。