Biomarkers of polyoma virus infection and lung cancer among non-smokers

描述（由申请人提供）：在美国，每年估计有16000 - 24000例肺癌病例发生在不吸烟者中（占总数的15-20%）。已知的环境风险因素只能解释这些病例中的一小部分。肺结核和慢性阻塞性肺病等炎症性疾病有助于肺癌的发生，并且有初步证据表明，已知或疑似致癌病毒的慢性感染可能是不吸烟者患肺癌的一个重要原因。多瘤病毒致癌作用的假设尚未在专门设计的流行病学研究中得到检验。本项目的总体目的是调查多瘤病毒感染标志物，特别是JC病毒和BK病毒与从不吸烟者肺癌之间的关系。血清学分析将使用经过验证的多重检测。一项回顾性病例对照研究对病例和对照进行了分析，初步结果令人鼓舞。该假设将在四项正在进行的前瞻性研究（其中两项来自亚洲）的458例从不吸烟的肺癌患者（以及相当数量的匹配对照）的血清样本中进行测试。血清样本的结果将在之前的病例对照研究中登记的72名肺癌患者中进行验证，并对其肿瘤样本进行分析。本研究将为不吸烟者多瘤病毒感染与肺癌之间的因果关系提供新的证据。确定癌症的传染原因为通过接种疫苗和根除癌症进行初级预防提供了机会。

DESCRIPTION (provided by applicant): An estimated 16,000-24,000 of lung cancer cases occur each year in the United States among never-smokers (15-20% of the total). Known environmental risk factors explain only a small proportion of these cases. Inflammatory conditions such as tuberculosis and chronic obstructive pulmonary disease contribute to lung carcinogenesis, and there is preliminary evidence that chronic infections with known or suspected oncogenic viruses may represent an important cause of lung cancer in never-smokers. The hypothesis of a carcinogenic role of polyoma viruses has not been tested in specifically designed epidemiologic studies. The overarching aim of this project is to investigate the association between markers of infection with polyoma viruses, and specifically JC virus and BK virus and lung cancer in never- smokers. A validated multiplex assay will be used for the serological analyses. Promising preliminary results have been obtained in the analysis of cases and controls from a retrospective case-control study. The hypothesis will be tested in serum samples of 458 never-smoking cases of lung cancer (and a comparable number of matched controls) from four on-going prospective studies, including two from Asia. The results of the serum samples will be validated arisen in series of 72 lung cancer patients who have been enrolled in a previous case-control study, from whom tumor samples will be analyzed. This study will provide novel evidence for or against a causal association between polyoma virus infection and lung cancer in never-smokers. The identification of infectious causes of cancer opens opportunities to primary prevention through vaccination and eradication.