Biorepository of cancer tissue samples from WTC responders

描述(由申请者提供)：世界贸易中心(WTC)响应人员的癌症发病率可能会增加，这一点一直令人担忧。然而，众所周知，大多数职业性癌症在致癌暴露后十年或十多年才显现出来；此外，该队列在组装时相对年轻。9/11事件十周年标志着对这一人群中癌症发生的研究具有重要的里程碑意义，因为暴露后的时间框架(10年)正在成为癌症的病因学相关因素，而队列正在老龄化，从而进入了生命中癌症发生的时间 变得更加频繁。现在有必要用癌症组织库来补充目前的流行病学癌症后续行动；生物谱学资源及其临床注释是推动转化研究的最强大的资源之一。我们建议为WTC新诊断的癌症实施组织库系统，重点是促进对这些肿瘤生物学的理解。该项目将启动和管理一个癌症组织样本和邻近正常组织的中央储存库，这些样本来自WTCHP参与者中诊断出的每个实体癌症。每种癌症的病理学和细胞学报告的相关信息也将集中存储。该存储库将具有以下功能 与纳入WTCHP时和在定期开展的后续行动中收集的参与者的临床、流行病学和暴露信息的主要WTCHP数据集相关联。随后，将从每个组织样本中制备RNA、DNA和组织微阵列(TMA)。这些样本将用于未来的基因组研究、基因与环境相互作用的研究以及DNA甲基化研究。还可以对储存的样本进行特殊分析，如免疫组织化学、原位杂交、DNA倍体分析、核形态计量学和荧光原位杂交。我们还将建立组织库作为科学界的资源，为符合条件的申请者定义一个程序，以请求用于研究用途的可用样本，并获取数据库中的流行病学和临床信息。组织库将代表必要的基础设施，以解决诸如特定致癌物暴露与某些癌症部位之间的联系、暴露可能与癌症有关的分子标记、WTC反应人员中肿瘤侵袭性的特定标记等问题。这最终将影响治疗方式，以及这些患者成功和存活的可能性。

DESCRIPTION (provided by applicant): There has been concern about the possibility of an increase in cancer incidence among the World Trade Center (WTC) responders. However, it is known that most occupational cancers become manifest only one or more decades after carcinogenic exposure; additionally, the cohort was relatively young at the time it was assembled. The tenth anniversary of 9/11 marks a significant landmark for studies on cancer occurrence in this population, since the time frame after the exposure (10 years) is becoming etiologically relevant for cancer, and the cohort is aging thus entering a time in life when cancer becomes more frequent. There is now a need to supplement the current epidemiologic cancer follow-up with a cancer tissue bank; biospecimen resources and their clinical annotations are among some of the most powerful resources fueling translational research. We propose to implement a tissue bank system for the WTC newly diagnosed cancers, focused on advancing the understanding of the biology of these tumors. This project will initiate and manage a central repository of cancer tissue samples and adjacent normal tissue from each solid cancer diagnosed among the WTCHP participants. Relevant information from pathology and cytology reports from each cancer will also be centrally stored. This repository will have the capability to be linked with the main WTCHP data set containing clinical, epidemiological and exposure information collected for the participants at the time of inclusion in the WTCHP and during the regularly performed follow up. Subsequently, RNA, DNA and tissue microarrays (TMA) will be prepared from each tissue sample. Such samples will be made available for future genomic research studies, studies of gene-environment interaction, as well as DNA methylation studies. Specific analyses such as immunohistochemistry, in situ hybridization, DNA ploidy analysis, nuclear morphometry, and fluorescent in situ hybridization will also be possible on the stored samples. We will also establish the tissue bank as a resource for the science community, by defining a process for qualified applicants to request available samples for research use, and for access epidemiologic and clinical information in the database. The tissue bank will represent the necessary infrastructure for addressing questions such as the link between specific carcinogens exposures and certain cancer sites, molecular signatures of exposure that could be linked to cancer, specific markers of tumor aggressiveness among WTC responders.. This will ultimately impact the modalities of treatment, and the probability of success and survival of these patients.