Core B

核心B通过提供最先进的RNAi工具来支持该计划中的每个项目。短发夹RNA（shRNA）是在该项目的支持下开发的，核心B和项目研究人员正在进行的创新有助于制造这些非常强大的生物工具。在过去的资助期间，核心设计了快速工程小鼠携带调控的shRNA表达盒的方法，设计了以多重方式功能验证shRNA的策略，并创建了第三代RNAi文库，对应于人类和小鼠中注释的蛋白质编码基因。在即将到来的请求支持期间，核心建议通过五个一般目标来帮助计划调查人员。首先，该计划将继续提供最先进的RNAi工具，用于分析单基因敲除或进行合并RNAi筛选。其次，核心将与研究人员合作，操作“传感器测定”，以确定针对感兴趣基因的最佳shRNA工具。第三，核心将针对计划研究人员感兴趣的基因组产生定制的RNAi文库。第四，核心将产生携带受调控的RNAi盒的小鼠，并帮助研究人员将这些与其他所需的遗传病变结合起来。最后，核心将继续努力改进RNAi技术，并将这些创新提供给该计划和整个社区。

Core B supports every project within the Program by providing access to state-of-the-art RNAi tools. Short hairpin RNAs (shRNAs) were developed with the support of this program, and ongoing innovations by Core B and Program investigators have helped to make these extremely powerful biological tools. During the past funding period, the Core devised methods to rapidly engineer mice carrying regulated shRNA expression cassettes, devised strategies for functionally validating shRNAs in a multiplexed fashion, and created a third generation of RNAi libraries corresponding to annotated protein coding genes in humans and mice. During the upcoming period of requested support, the core proposes to aid Program investigators through five general aims. First, the program will continue to provide access to state-of-the-art RNAi tools for analyzing either single gene knockdowns or for performing pooled RNAi screens. Second, the Core will work with investigators to operate the "sensor assay" to identify optimal shRNAs tools against genes of interest. Third, the core will produce custom RNAi libraries against sets of genes that are of interest to Program investigators. Fourth, the Core will produce mice carrying regulated RNAi cassettes and aid investigators in combining these with other desired genetic lesions. Finally, the Core will carry on its efforts to improve RNAi technologies and make those innovations available to the Program and to the community at large.