Core B

Core B通过提供最先进的RNAi工具来支持程序中的每个项目。短发夹rna （shrna）是在这个项目的支持下开发出来的，而Core B和项目研究人员正在进行的创新帮助制造了这些极其强大的生物工具。在过去的资助期间，Core设计了快速改造携带受调控shRNA表达磁带的小鼠的方法，设计了以多路复用方式对shRNA进行功能验证的策略，并创建了与人类和小鼠中注释蛋白编码基因相对应的第三代RNAi文库。在即将到来的请求支助期间，核心建议通过五个总体目标来帮助方案调查员。首先，该项目将继续提供最先进的RNAi工具，用于分析单基因敲低或执行集合RNAi筛选。其次，Core将与研究人员合作，操作“传感器试验”，以确定针对感兴趣基因的最佳shrna工具。第三，核心将针对程序研究者感兴趣的基因集生成定制的RNAi文库。第四，Core将生产携带调控RNAi磁带的小鼠，并帮助研究人员将这些磁带与其他所需的遗传病变结合起来。最后，核心将继续努力改进RNAi技术，并将这些创新成果提供给项目和整个社区。

Core B supports every project within the Program by providing access to state-of-the-art RNAi tools. Short hairpin RNAs (shRNAs) were developed with the support of this program, and ongoing innovations by Core B and Program investigators have helped to make these extremely powerful biological tools. During the past funding period, the Core devised methods to rapidly engineer mice carrying regulated shRNA expression cassettes, devised strategies for functionally validating shRNAs in a multiplexed fashion, and created a third generation of RNAi libraries corresponding to annotated protein coding genes in humans and mice. During the upcoming period of requested support, the core proposes to aid Program investigators through five general aims. First, the program will continue to provide access to state-of-the-art RNAi tools for analyzing either single gene knockdowns or for performing pooled RNAi screens. Second, the Core will work with investigators to operate the "sensor assay" to identify optimal shRNAs tools against genes of interest. Third, the core will produce custom RNAi libraries against sets of genes that are of interest to Program investigators. Fourth, the Core will produce mice carrying regulated RNAi cassettes and aid investigators in combining these with other desired genetic lesions. Finally, the Core will carry on its efforts to improve RNAi technologies and make those innovations available to the Program and to the community at large.