Modulation of Gene Expression Through RNAi

通过 RNAi 调节基因表达

• 批准号: 8234421
• 负责人: Gregory J Hannon
• 金额: $ 36.49万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8234421
• 关键词: Animals; Biological; Biological Assay; Code; Collaborations; Communities; Cultured Cells; Custom; Development; Engineering; Funding; Gene Expression; Generations; Genes; Genetic; Genome; Goals; Human; Individual; Laboratories; Lesion; Libraries; Methodology; Methods; Mus; NCI Center for Cancer Research; Phenotype; Proteins; Protocols documentation; RNA Interference; RNA Polymerase II; Research Personnel; Technology; Work; base; cell type; improved; innovation; interest; member; mouse model; programs; promoter; response; scaffold; sensor; small hairpin RNA; tool

Core B supports every project within the Program by providing access to state-of-the-art RNAi tools. Short hairpin RNAs (shRNAs) were developed with the support of this program, and ongoing innovations by Core B and Program investigators have helped to make these extremely powerful biological tools. During the past funding period, the Core devised methods to rapidly engineer mice carrying regulated shRNA expression cassettes, devised strategies for functionally validating shRNAs in a multiplexed fashion, and created a third generation of RNAi libraries corresponding to annotated protein coding genes in humans and mice. During the upcoming period of requested support, the core proposes to aid Program investigators through five general aims. First, the program will continue to provide access to state-of-the-art RNAi tools for analyzing either single gene knockdowns or for performing pooled RNAi screens. Second, the Core will work with investigators to operate the "sensor assay" to identify optimal shRNAs tools against genes of interest. Third, the core will produce custom RNAi libraries against sets of genes that are of interest to Program investigators. Fourth, the Core will produce mice carrying regulated RNAi cassettes and aid investigators in combining these with other desired genetic lesions. Finally, the Core will carry on its efforts to improve RNAi technologies and make those innovations available to the Program and to the community at large.

Core B通过提供最先进的RNAi工具来支持程序中的每个项目。短