Modulation of Gene Expression Through RNAi
通过 RNAi 调节基因表达
基本信息
- 批准号:8234421
- 负责人:
- 金额:$ 36.49万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2012
- 资助国家:美国
- 起止时间:2012-01-01 至 2016-12-31
- 项目状态:已结题
- 来源:
- 关键词:AnimalsBiologicalBiological AssayCodeCollaborationsCommunitiesCultured CellsCustomDevelopmentEngineeringFundingGene ExpressionGenerationsGenesGeneticGenomeGoalsHumanIndividualLaboratoriesLesionLibrariesMethodologyMethodsMusNCI Center for Cancer ResearchPhenotypeProteinsProtocols documentationRNA InterferenceRNA Polymerase IIResearch PersonnelTechnologyWorkbasecell typeimprovedinnovationinterestmembermouse modelprogramspromoterresponsescaffoldsensorsmall hairpin RNAtool
项目摘要
Core B supports every project within the Program by providing access to state-of-the-art RNAi tools. Short
hairpin RNAs (shRNAs) were developed with the support of this program, and ongoing innovations by Core
B and Program investigators have helped to make these extremely powerful biological tools. During the past
funding period, the Core devised methods to rapidly engineer mice carrying regulated shRNA expression
cassettes, devised strategies for functionally validating shRNAs in a multiplexed fashion, and created a third
generation of RNAi libraries corresponding to annotated protein coding genes in humans and mice. During
the upcoming period of requested support, the core proposes to aid Program investigators through five
general aims. First, the program will continue to provide access to state-of-the-art RNAi tools for analyzing
either single gene knockdowns or for performing pooled RNAi screens. Second, the Core will work with
investigators to operate the "sensor assay" to identify optimal shRNAs tools against genes of interest. Third,
the core will produce custom RNAi libraries against sets of genes that are of interest to Program
investigators. Fourth, the Core will produce mice carrying regulated RNAi cassettes and aid investigators in
combining these with other desired genetic lesions. Finally, the Core will carry on its efforts to improve RNAi
technologies and make those innovations available to the Program and to the community at large.
Core B通过提供最先进的RNAi工具来支持程序中的每个项目。短
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Gregory J Hannon其他文献
Free energy lights the path toward more effective RNAi
自由能照亮了通向更有效的 RNAi 的道路
- DOI:
10.1038/ng1203-303 - 发表时间:
2003-12-01 - 期刊:
- 影响因子:29.000
- 作者:
Jose M Silva;Ravi Sachidanandam;Gregory J Hannon - 通讯作者:
Gregory J Hannon
Gregory J Hannon的其他文献
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{{ truncateString('Gregory J Hannon', 18)}}的其他基金
An optogenetic toolkit for the interrogation and control of single cells.
用于询问和控制单细胞的光遗传学工具包。
- 批准号:
8822629 - 财政年份:2014
- 资助金额:
$ 36.49万 - 项目类别:
Acquisition of a high-throughput compute cluster for biological data analysis
获取用于生物数据分析的高通量计算集群
- 批准号:
8247532 - 财政年份:2012
- 资助金额:
$ 36.49万 - 项目类别:
A ROLE FOR THE P-BODY COMPONENT GW182 IN MICRORNA FUNCTION
P 体成分 GW182 在 MICRORNA 功能中的作用
- 批准号:
8171361 - 财政年份:2010
- 资助金额:
$ 36.49万 - 项目类别:
Cold Spring Harbor Laboratory Cancer Research Center
冷泉港实验室癌症研究中心
- 批准号:
7910927 - 财政年份:2009
- 资助金额:
$ 36.49万 - 项目类别:
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