human Microvessel Culture System (hMCS)

描述（由申请人提供）：人类微血管培养系统（hMCS）血管生成靶向疗法，抑制或促进新血管生长，具有治疗多种疾病的潜力，包括癌症，眼病，心脏和周围血管疾病，皮肤病，纤维增生性疾病和炎症性疾病，如关节炎。此外，越来越多的证据表明，针对多种不同血管活动的药物组合被证明对许多这些疾病更有效。随着对这些治疗的需求和复杂性的增加，对相关血管生长和稳定性的更多信息分析的需求日益增长。理想情况下，这样的分析应该尽可能多地概括体内生物过程，同时保持实验的简单性和成本效益。血管组学公司建立在成熟的技术基础上，该技术基于从各种组织中分离的微血管的操作和培养。利用啮齿类动物资源，这种微血管培养系统已经被广泛有效地用作各种血管相关活动和治疗的更全面的实验和发现平台。由于该系统利用了完整的微血管，因此在培养和体内环境中都能更准确地再现血管生成。在这个系统中，真正的新血管在三维基质环境中从完整的微血管中萌发和生长。因此，所有血管生成相关的活动/元素，包括血管周围细胞的行为，都存在并整合在分析中。本建议的总体目标是

DESCRIPTION (provided by applicant): Human Microvessel Culture System (hMCS) Angiogenesis-targeting therapies, either inhibiting or promoting new vessel growth, have the potential for treating numerous diseases including cancer, eye diseases, cardiac and peripheral vascular disease, skin disorders, fibro-proliferative diseases, and inflammatory conditions such as arthritis. Furthermore, growing evidence indicates that for many of these diseases combinations of agents targeting multiple different vascular activities are proving more efficacious. As demand and complexity increases for these therapies, there is a growing need for more informative assays of relevant vascular growth and stability. Ideally, such an assay should recapitulate as much of the in vivo biological process as possible, while maintaining experimental simplicity and cost effectiveness. Angiomics, Inc. was founded on proven technology based on the manipulation and culturing of isolated microvessels from a variety of tissues. Utilizing rodent sources, this microvessel culture system has been used extensively and effectively as a more comprehensive experimental and discovery platform for a variety of vascular-related activities and therapies. Because the system utilizes intact microvessels, angiogenesis is more accurately recapitulated in both the culture and in vivo settings. In this system, true neovessels sprout and grow from intact microvessels within a 3-D matrix environment. Consequently, all angiogenesis-relevant activities/elements including perivascular cell behavior are present and integrated in the assay. The overall objective of this proposal is to adapt this proven and versatile microvessel culture system to support human microvessel viability and activity to add utility and value to the platform. It is guided by two Objectives tha 1) identify media components, guided by the rodent system, and 2) evaluate combinations of candidate growth factors or hypoxia factors that will support human microvessel viability and angiogenesis in culture.