Cell and Tissue Imaging Core

这一核心的总体目标是提供基础设施和支持， 用显微镜检查含有生物制剂的细胞和组织标本， 细胞或组织样品可以在密闭环境中保存，直到细胞或组织样品处于中和和非感染性状态。细胞 NEIDL的组织成像核心（CTIC）将提供一系列成像系统， 用最先进的技术制作标本几个高分辨率的可用性 显微镜平台将使NEIDL研究人员能够从多探针、活细胞 分析。这种基础设施将帮助用户了解宿主细胞/病原体相互作用， 允许对实验感染动物的感染组织进行有针对性的病理学评估。 了解三维细胞和细胞器的断层扫描，相互连接和形态 (3D)在活的感染组织中对于了解体内行为是非常宝贵的， 传染性病原体的发病机理。 CITC的业务目标是： a.提供基础设施和专业知识，以可视化其细胞中的BSL-4病原体 在固定的细胞、组织和动物中的背景。 B.提供基础设施和专业知识，以可视化其细胞中的BSL-4病原体 随着时间的推移，在固定的细胞，组织和动物中的背景。 C.与NEIDL科学核心互动和合作，并为研究提供支持 处理NIAID A-C类优先病原体的项目。 D.对核心人员和NEIDL附属研究人员进行生物成像技术培训。

The overall goal of this core is to provide the infrastructure and support to macroscopically and microscopically examine cells and tissue specimens containing biological agents that require high containment until the cell or tissue samples are in a neutralized and non-infectious state. The Cell and Tissue Imaging Core (CTIC) at the NEIDL will offer a range of imaging systems to analyze specimens using state-of-the-art technologies. The availability of several high resolution microscopy platforms will allow NEIDL investigators to gather information from multi-probe, live cell analyses. Such infrastructure will help users to understand the host cell/pathogen interactions and permit targeted, pathological assessment of infected tissues from experimentally infected animals. Knowing the tomography, interconnectivity and morphology of cells and organelles in threedimensions (3D) in living infected tissues is invaluable for understanding the in vivo behavior and pathogenesis of infectious agents. The operational goals of CITC are: a. To provide the infrastructure and expertise to visualize BSL-4 pathogens in their cellular context in fixed cells, tissues and animals. b. To provide the infrastructure and expertise to visualize BSL-4 pathogens in their cellular context in fixed cells, tissues, and animals over time. c. To interact and collaborate with NEIDL scientific cores and to provide support to research projects dealing with NIAID category A-C priority pathogens. d. To train core personnel and NEIDL-affiliated researchers in bioimaging techniques.