Cell and Tissue Imaging Core

这一核心的总体目标是为宏观和 显微镜检查含有生物制剂的细胞和组织样本，这些生物制剂需要 遏制，直到细胞或组织样本处于中和和非感染状态。《细胞》 Neidl的组织成像核心(CTIC)将提供一系列成像系统进行分析 使用最先进技术的标本。几种高分辨率产品的可用性 显微镜平台将允许Neidl的研究人员从多探针、活细胞中收集信息 分析。这样的基础设施将帮助用户了解宿主细胞/病原体的相互作用和 允许对实验感染动物的感染组织进行有针对性的病理评估。 了解三维细胞和细胞器的断层扫描、互连和形态 (3D)在受感染的活体组织中对了解体内行为和 感染性病原体的发病机制。 国贸中心的业务目标是： A.提供基础设施和专业知识，以可视化其细胞中的BSL-4病原体 在固定细胞、组织和动物中的上下文。 B.提供基础设施和专业知识，以可视化其细胞内的BSL-4病原体 随着时间的推移，固定细胞、组织和动物中的上下文。 C.与Neidl科学核心进行互动和合作，并为研究提供支持 处理NIAID A-C类优先病原体的项目。 D.对核心人员和Neidl附属研究人员进行生物成像技术培训。

The overall goal of this core is to provide the infrastructure and support to macroscopically and microscopically examine cells and tissue specimens containing biological agents that require high containment until the cell or tissue samples are in a neutralized and non-infectious state. The Cell and Tissue Imaging Core (CTIC) at the NEIDL will offer a range of imaging systems to analyze specimens using state-of-the-art technologies. The availability of several high resolution microscopy platforms will allow NEIDL investigators to gather information from multi-probe, live cell analyses. Such infrastructure will help users to understand the host cell/pathogen interactions and permit targeted, pathological assessment of infected tissues from experimentally infected animals. Knowing the tomography, interconnectivity and morphology of cells and organelles in three-dimensions (3D) in living infected tissues is invaluable for understanding the in vivo behavior and pathogenesis of infectious agents. The operational goals of CITC are: a. To provide the infrastructure and expertise to visualize BSL-4 pathogens in their cellular context in fixed cells, tissues and animals. b. To provide the infrastructure and expertise to visualize BSL-4 pathogens in their cellular context in fixed cells, tissues, and animals over time. c. To interact and collaborate with NEIDL scientific cores and to provide support to research projects dealing with NIAID category A-C priority pathogens. d. To train core personnel and NEIDL-affiliated researchers in bioimaging techniques.