Studying the oviposition of Anopheles gambiae: the development of novel tools for

研究冈比亚按蚊的产卵：开发新工具

• 批准号: 8515268
• 负责人: Steve Lindsay
• 金额: $ 41.75万
• 依托单位: UNIVERSITY OF DURHAM
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-24 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8515268
• 关键词: Adult; Africa; Africa South of the Sahara; Anopheles gambiae; Area; Bacteria; Behavior; Behavioral; Bite; Breeding; Chemicals; Child; Clinical; Communities; Complement; Culicidae; Development; Dose; Drug Formulations; Ecology; Environment; Epidemiology; Female; Fertility; Film; Future; Generations; Habitats; Infection; Insecticides; Intervention; Invertebrates; Juvenile Hormones; Kenya; Knowledge; Laboratories; Larva; Lead; Longevity; Malaria; Measures; Methods; Monitor; Oviposition; Patient currently pregnant; Performance; Population; Predisposition; Recording of previous events; Reporting; Research; Residual state; Resistance; Resources; Rest; Retreatment; Risk; Sampling; Seasons; Semiochemicals; Silicon; Site; Staging; Surface; Sweden; Testing; Time; Water; base; chemical release; cost; cost effective; effective therapy; efficacy testing; egg; improved; innovation; interest; killings; larval control; novel; offspring; olfactory stimulus; preference; programs; pyrethroid; response; tool; transmission process; vector; vector control; visual stimulus

Sub-Saharan Africa bares the brunt of the world's malaria burden, with nearly 80% of all cases occurring in this region. However, we are now at a turning point in the history of malaria control in Africa. For the first time in a generation reports of malaria declining have become common due to high coverage with long-lasting insecticide treated nets and the prompt and effective treatment of clinical cases. In order to maintain these hard won gains and to make further reductions in malaria transmission it is important to improve and develop new strategies for vector control, which will be applicable in different environments, affordable, and complement existing interventions, so that greater reductions in malaria transmission can be achieved. This project aims to develop more efficient and cost-effective methods for controlling and monitoring Anopheles gambiae populations, the major vector of malaria in Africa. This approach is underpinned by multi-disciplinary research to identify the visual and olfactory stimuli that cause female An. gambiae to lay eggs at certain sites and not others. Such knowledge will be used to (1) identify important breeding sites and selectively treat preferred sites with novel, long-lasting and environmentally friendly larvicides and (2) provide a rational basis for developing traps to monitor adult mosquitoes. Specifically we propose to test: (1) if bacteria-generated volatiles from aquatic habitats function as oviposition semiochemicals; (2) whether the bacterial communities and chemicals released from and present in stagnant water bodies differ between sites with and without An. gambiae larvae, (3) test novel residual larvicides (pyriproxyfen, an insect growth regulator and a silicon-based monomolecular surface film) against An. gambiae and (5) develop an oviposition trap for collecting gravid An. gambiae. Therefore, the performance of two relatively novel agents, will be evaluated under laboratory and field conditions and their potential in an `attract-and-kill' strategy combining findings from all specific objectives tested. Most of these studies will be carried out in the laboratory, in enclosed greenhouses and in the field in Kenya. However, some of the detailed chemical analysis will need to be carried out in specialised laboratories in Sweden. The findings from this study may lead to the development of new sampling and control tools for malaria vectors. Such tools would be valuable additions to an Integrated Vector Management package for the control of malaria in sub-Saharan Africa.

撒哈拉以南非洲是世界疟疾负担的主要地区， 发生在这个地区。然而，我们现在正处于疟疾控制历史的转折点， 非洲这是一代人以来疟疾下降的报告第一次普遍， 长效驱虫蚊帐的高覆盖率以及及时有效地处理 临床病例为了保持这些来之不易的成果并进一步减少疟疾， 因此，必须改进和制定新的病媒控制战略， 适用于不同的环境，负担得起，并补充现有的干预措施， 减少疟疾传播是可以实现的。该项目旨在开发更高效、 控制和监测冈比亚按蚊种群的成本效益高的方法， 非洲疟疾的传播媒介。这种方法得到多学科研究的支持，以确定 视觉和嗅觉的刺激，导致女性安。冈比亚在某些地点产卵，而不是其他地点。 这些知识将用于（1）确定重要的繁殖地点，并选择性地处理首选的 使用新型、长效和环保的杀幼虫剂的地点，以及（2）为以下方面提供合理依据： 发展监测成年蚊子的诱捕器。具体而言，我们建议测试：（1）如果细菌产生 水生环境挥发物作为产卵信息化合物的功能;（2）细菌是否 不同地点之间，从停滞水体中释放和存在的群落和化学物质不同 有没有安冈比亚幼虫，（3）测试新的残留杀幼虫剂（吡丙醚，一种昆虫生长 调节剂和硅基单分子表面膜）对An的影响。冈比亚和（5）发展一个 一种收集怀孕按蚊的产卵诱捕器。冈比亚。因此，两种表现形式比较新颖 将在实验室和实地条件下对其“引诱和杀灭”的潜力进行评估。 综合所有具体目标测试结果的战略。 这些研究大部分将在实验室、封闭温室和田间进行， 肯尼亚.然而，一些详细的化学分析将需要在专门的 瑞典的实验室这项研究的结果可能会导致新的抽样的发展 以及疟疾病媒的控制工具。这些工具将是对综合矢量的宝贵补充 撒哈拉以南非洲疟疾控制管理一揽子计划。

项目成果

Field evaluation of two novel sampling devices for collecting wild oviposition site seeking malaria vector mosquitoes: OviART gravid traps and squares of electrocuting nets.

• DOI: 10.1186/s13071-016-1557-7
• 发表时间: 2016-05-10
• 期刊: Parasites & vectors
• 影响因子: 3.2
• 作者: Dugassa S;Lindh JM;Lindsay SW;Fillinger U
• 通讯作者: Fillinger U

Electric nets and sticky materials for analysing oviposition behaviour of gravid malaria vectors.

• DOI: 10.1186/1475-2875-11-374
• 发表时间: 2012-11-14
• 期刊: Malaria journal
• 影响因子: 3
• 作者: Dugassa S;Lindh JM;Torr SJ;Oyieke F;Lindsay SW;Fillinger U
• 通讯作者: Fillinger U

Larval source management for malaria control in Africa: myths and reality.

• DOI: 10.1186/1475-2875-10-353
• 发表时间: 2011-12-13
• 期刊: Malaria journal
• 影响因子: 3
• 作者: Fillinger U;Lindsay SW
• 通讯作者: Lindsay SW

Analysing the oviposition behaviour of malaria mosquitoes: design considerations for improving two-choice egg count experiments.

分析疟疾蚊子的产卵行为：改进二选卵计数实验的设计考虑。

• DOI: 10.1186/s12936-015-0768-2
• 发表时间: 2015
• 期刊: Malaria journal
• 影响因子: 3
• 作者: Okal,MichaelN;Lindh,JennyM;Torr,SteveJ;Masinde,Elizabeth;Orindi,Benedict;Lindsay,SteveW;Fillinger,Ulrike
• 通讯作者: Fillinger,Ulrike

Development of a gravid trap for collecting live malaria vectors Anopheles gambiae s.l.

• DOI: 10.1371/journal.pone.0068948
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Dugassa S;Lindh JM;Oyieke F;Mukabana WR;Lindsay SW;Fillinger U
• 通讯作者: Fillinger U