Protein-protein interactions in the transcriptional regulation of neural development

神经发育转录调节中的蛋白质-蛋白质相互作用

• 批准号: DP140102318
• 负责人: Prof Jacqueline Matthews
• 金额: $ 25.38万
• 依托单位: The University of Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP140102318
• 关键词: Protein; protein; interactions; transcriptional; regulation

Combinations of transcription factor protein regulate the expression of genes, but just how different combinations of these proteins specifically up or down regulate specific genes is poorly understood. This project will focus on the LIM-homeodomain transcription factors, which act in combination to form the so-called "LIM code" that specifies cell type specification during development. Using a series of biophysical and structural approaches. This project will unravel the networks of protein-protein interactions that form the basis of the LIM code. This will help address a major issue in the field: How do interactions between transcription factors regulate DNA-binding specificity. Ultimately, this work may also lead to regeneration of nerve tissue.

转录因子蛋白的组合调节基因的表达，但人们对这些蛋白质的不同组合如何特异性上调或下调特定基因的了解甚少。该项目将重点关注 LIM 同源域转录因子，它们联合作用形成所谓的“LIM 代码”，在开发过程中指定细胞类型规范。使用一系列生物物理和结构方法。该项目将揭示构成 LIM 代码基础的蛋白质-蛋白质相互作用网络。这将有助于解决该领域的一个主要问题：转录因子之间的相互作用如何调节 DNA 结合特异性。最终，这项工作还可能导致神经组织的再生。