Mechanisms of gene regulation

本项目旨在确定特定基因打靶的分子基础。转录因子复合体通过在特定位置与DNA结合、修饰和环化染色质、招募基本转录机制来调节基因的表达。以血细胞转录因子复合体为模型，该项目将揭示微调DNA结合的一组蛋白质之间的相互作用，并招募调节基因表达的辅助蛋白质。所提供的机制细节有望为生物技术应用中人工上调或下调基因提供信息，并最终治疗具有遗传成分的疾病。

This project aims to determine the molecular basis of specific gene targeting. Transcription factor complexes regulate gene expression by binding to DNA at specific sites, modifying and looping chromatin, and recruiting the basal transcription machinery. Using blood cell transcription factor complexes as a model, this project will reveal interactions between sets of proteins that fine-tune DNA binding and recruit accessory proteins that regulate gene expression. The mechanistic detail provided is expected to inform the artificial up- or down-regulation of genes in biotechnological applications and ultimately treat disease which have a genetic component.